接受肠道膀胱扩大术并经L-赖氨酸治疗的大鼠肠道上皮中的移行化生

Transitional metaplasia in intestinal epithelium of rats submitted to intestinal cystoplasty and treatment with L -lysine.

作者信息

Santos Alessandra Marques Dos, Coelho Joao Paulo Ferreira, Juanes Camila de Carvalho, Azevedo Rafael Barbosa de, Diniz Clara Araujo, Jamacaru Francisco Vagnaldo Fechine, Dornelas Conceição Aparecida

机构信息

Fellow Master degree, Postgraduate Program in Pathology, Department of Pathology, Universidade Federal do Ceará (UFC), Brazil. Intellectual and scientific content of the study, histopathological examinations, technical procedures, manuscript preparation.

Graduate student, Scientific Iniciation Program, UFC, Fortaleza-CE, Brazil. Technical procedures.

出版信息

Acta Cir Bras. 2017 Apr;32(4):297-306. doi: 10.1590/s0102-865020170040000297.

DOI:10.1590/s0102-865020170040000297

PMID:28538804

Abstract

PURPOSE

: To evaluated the effects of L-lysine on the intestinal and urothelial epithelia in cystoplasty in rats.

METHODS

: Twenty-eight 9-week-old rats were assigned to 4 groups: Group A (n=8) cystoplasty followed by administration of L-lysine (150 mg/kg body weight by gavage) for 30 weeks; Group B (n=8) cystoplasty + water for 30 weeks; Group C (n=6) L-lysine for 30 weeks; Group D (n=6) water for 30 weeks.

RESULTS

: On histopathology with hematoxylin and eosin, mild to moderate hyperplasia transitional was observed in at the site of anastomosis in all animals submitted to cystoplasty (Groups A and B), but "transitional metaplasia" of the intestinal glandular epithelium was more accentuated in Group A (p=0.045). No inflammatory cells, dysplasia or abnormalities were observed. Staining with Alcian blue revealed a substantial reduction of goblet cells and mucins in the colon segment (Groups A and B).

CONCLUSION

: The administration of L-lysine to rats accelerated the development of transitional metaplasia in the epithelium of the colon segment in cystoplasty.

摘要