MOE Key Laboratory of Bioinformatics and Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084, China.
Animal Facility of the Center of Biomedical Analysis, Tsinghua University,Shuangqing Road 30, HaiDian district, Beijing 100084, China.
Eur J Cell Biol. 2017 Sep;96(6):567-578. doi: 10.1016/j.ejcb.2017.05.003. Epub 2017 May 17.
Adipose tissue is a complicated organ that not only stores excess energy, but also secrets many adipokines regulating whole-body energy hemostasis. Dysfunction of adipose tissue leads to metabolic disorders such as insulin resistance, hypertension, cardiovascular diseases. In this study, we generated a mouse model with overexpression of Angiotensin II type 1 receptor-associated protein (ATRAP) in adipose tissue specifically. Under a normal diet, ATRAP transgene (TgATRAP) mice showed similar bodyweight, fat mass and insulin sensitivity with wild-type controls (WT). When challenged with a high fat diet, TgATRAP mice ameliorated insulin sensitivity, decreased fat mass compared with WT. Morphology and gene expression of adipose tissue, indicated that adipogenesis, adipocyte browning and angiogenesis of adipose tissue were increased in TgATRAP mice. Overexpression of ATRAP induced adiponectin expression both in adipose tissue and primary adipocyte. Our data revealed that adipose ATRAP plays an important role in preventing metabolic disorders and adiponectin possibly mediates the effects of adipose ATRAP.
脂肪组织是一种复杂的器官，不仅储存多余的能量，还分泌许多调节全身能量平衡的脂肪因子。脂肪组织功能障碍会导致代谢紊乱，如胰岛素抵抗、高血压、心血管疾病。在这项研究中，我们构建了脂肪组织特异性过表达血管紧张素 II 型 1 型受体相关蛋白 (ATRAP) 的小鼠模型。在正常饮食下，ATRAP 转基因 (TgATRAP) 小鼠的体重、脂肪量和胰岛素敏感性与野生型对照 (WT) 相似。当用高脂肪饮食挑战时，与 WT 相比，TgATRAP 小鼠改善了胰岛素敏感性，减少了脂肪量。脂肪组织的形态和基因表达表明，TgATRAP 小鼠的脂肪生成、脂肪细胞棕色化和血管生成增加。ATRAP 的过表达诱导脂肪组织和原代脂肪细胞中脂联素的表达。我们的数据表明，脂肪组织中的 ATRAP 在预防代谢紊乱中起着重要作用，而脂联素可能介导脂肪组织 ATRAP 的作用。
