Hughes H, Nowlin J, Gaskell S J, Parr V C
Center for Experimental Therapeutics, Baylor College of Medicine, Houston, Texas 77030.
Biomed Environ Mass Spectrom. 1988 Oct;16(1-12):409-13. doi: 10.1002/bms.1200160180.
Gas chromatography/electron capture negative ion mass spectrometry of eicosanoids, as pentafluorobenzyl (PFB) ester, methyl oxime (where applicable), trimethylsilyl (TMS) ether derivatives, is reported using a double-focusing instrument of trisector configuration. Sub-picogram detection limits were observed for prostaglandins E1, E2 and F2 alpha during selected ion monitoring (SIM) of [M-PFB]- ions. Selected reaction monitoring (SRM) of [M-PFB]-----[M-PFB-TMSOH]-, occurring in the first field-free region, was of modest sensitivity, reflecting the stability of the [M-PFB]- ions. The leukotriene B4 (LTB4) derivative was successfully analyzed by SIM at the low-picogram level. In this instance, the fragmentation [M-PFB]-----[M-PFB-TMSOH] occurred in high yield in the first field-free region. The advantageous improvement in selectivity of detection that may be achieved with SRM was evident during the analysis of a serum extract for LTB4.
本文报道了使用扇形磁场双聚焦仪器，对作为五氟苄基（PFB）酯、甲基肟（如适用）、三甲基硅烷基（TMS）醚衍生物的类二十烷酸进行气相色谱/电子捕获负离子质谱分析。在对[M - PFB]-离子进行选择离子监测（SIM）时，观察到前列腺素E1、E2和F2α的检测限低于皮克级。在第一个无场区发生的[M - PFB]-----[M - PFB - TMSOH]-的选择反应监测（SRM）灵敏度适中，这反映了[M - PFB]-离子的稳定性。白三烯B4（LTB4）衍生物通过SIM在低皮克级水平上成功进行了分析。在这种情况下，[M - PFB]-----[M - PFB - TMSOH]的裂解在第一个无场区以高产率发生。在分析血清提取物中的LTB4时，SRM在检测选择性方面所带来的有利改进是显而易见的。
