Qin Xijun, Ma Xiaojun, Tu Dongping, Luo Zuliang, Huang Jie, Mo Changming
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China.
Guangxi University of Chinese Medicine, Nanning 530200, China.
Acta Pharm Sin B. 2017 May;7(3):381-389. doi: 10.1016/j.apsb.2016.11.004. Epub 2017 Mar 14.
The 5-HT receptor agonist 8-hydroxy-2-[di--propylamino] tetralin (8-OH-DPAT) promotes ejaculation of male rats, whereas dapoxetine delays this process. However, the gene expression profile of the brain at ejaculation following administrationof these two compounds has not been fully elucidated. In the present study, a transcriptomic BodyMap was generated by conducting mRNA-Seq on brain samples of male Sprague-Dawley rats. The study included four groups: pre-copulatory control (CK) group, ejaculation (EJ) group, 0.5 mg/kg 8-OH-DPAT-ejaculation group (DPAT), and 60 mg/kg dapoxetine-ejaculation (DAP) group. The resulting analysis generated an average of approximately 47 million sequence reads. Significant differences in the gene expression profiles of the aforementioned groups were observed in the EJ (257 genes), DPAT (349 genes) and the DAP (207 genes) compared with the control rats. The results indicate that the expression of and was significantly different after treatment with 8-OH-DPAT, whereas the expression of was significantly different after treatment with dapoxetine. Other genes, such as , and , exhibited significant differences in expression after the two treatments and are related to bladder cancer, renal cell carcinoma and sexual addiction. The present study reveals the basic pattern of gene expression that was activated at ejaculation in the presence of 8-OH-DPAT or dapoxetine, providing preliminary gene expression information during rat ejaculation.
5-羟色胺受体激动剂8-羟基-2-[二丙基氨基]四氢萘（8-OH-DPAT）可促进雄性大鼠射精，而达泊西汀则延迟这一过程。然而，这两种化合物给药后射精时大脑的基因表达谱尚未完全阐明。在本研究中，通过对雄性Sprague-Dawley大鼠的脑样本进行mRNA测序生成了转录组体图谱。该研究包括四组：交配前对照组（CK）、射精组（EJ）、0.5mg/kg 8-OH-DPAT射精组（DPAT）和60mg/kg达泊西汀射精组（DAP）。结果分析平均产生了约4700万个序列读数。与对照大鼠相比，在EJ组（257个基因）、DPAT组（349个基因）和DAP组（207个基因）中观察到上述组基因表达谱的显著差异。结果表明，8-OH-DPAT处理后，[具体基因1]和[具体基因2]的表达有显著差异，而达泊西汀处理后，[具体基因3]的表达有显著差异。其他基因，如[具体基因4]、[具体基因5]和[具体基因6]，在两种处理后表达有显著差异，且与膀胱癌、肾细胞癌和性成瘾有关。本研究揭示了在8-OH-DPAT或达泊西汀存在下射精时激活的基因表达基本模式，提供了大鼠射精过程中的初步基因表达信息。
