Cunha Joana T, Domingues Lucília
CEB-Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
Methods Mol Biol. 2017;1620:183-193. doi: 10.1007/978-1-4939-7060-5_13.
Food safety and quality are nowadays a major consumers' concern. In the dairy industry the fraudulent addition of cheaper/lower-quality milks from nonlegitimate species/breeds compromises the quality and value of the final product. Despite the already existing approaches for identification of the species origin of milk, there is little information regarding differentiation at an intra-species level. In this protocol we describe a low-cost, sensitive, fast, and reliable analytical technique-Random Amplified Polymorphic DNA/Sequence Characterized Amplified Region (RAPD/SCAR)-capable of an efficient detection of adulterant breeds in milk mixtures used for fraudulent manufacturing of dairy products and suitable for the detection of milk adulteration in processed dairy foods.
如今，食品安全与质量是消费者主要关心的问题。在乳制品行业，从不合法的物种/品种中欺诈性添加更便宜/质量更低的牛奶会损害最终产品的质量和价值。尽管已有识别牛奶物种来源的方法，但关于种内水平差异的信息却很少。在本方案中，我们描述了一种低成本、灵敏、快速且可靠的分析技术——随机扩增多态性DNA/序列特征扩增区域（RAPD/SCAR），它能够有效检测用于欺诈性生产乳制品的牛奶混合物中的掺假品种，并且适用于检测加工乳制品中的牛奶掺假情况。
