Janzen E G, Jandrisits L T, Barber D L
Department of Chemistry and Biochemistry, Guelph-Waterloo Centre for Graduate Work in Chemistry, University of Guelph, Ontario, Canada.
Free Radic Res Commun. 1987;4(2):115-23. doi: 10.3109/10715768709088096.
The spin trap 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), is commonly used for the detection of short-lived free radicals produced by neutrophils during their respiratory burst. The spin adducts of both the superoxide ion (O2-) and the hydroxyl radical (.OH) are detectable during this process. Since myeloperoxidase (MPO), which is also active during the respiratory burst, produces hypochlorous acid (HOCl) (HOCl) in the presence of chloride ions (Cl-) and hydrogen peroxide (H2O2), this species has been investigated as a possible source of the DMPO-OH adduct. At concentrations of hypochlorous acid between 0.1 and 0.7 mumol/ml the DMPO-OH spin adduct is detected using electron spin resonance (ESR) techniques. Two possible mechanisms for the formation of this adduct are proposed. These findings suggest that the product of MPO, namely hypochlorous acid, is a possible source of the hydroxyl spin adduct detected during the respiratory burst.
自旋捕获剂5,5-二甲基-1-吡咯啉-N-氧化物（DMPO）通常用于检测中性粒细胞在呼吸爆发过程中产生的短寿命自由基。在此过程中，超氧阴离子（O2-）和羟基自由基（·OH）的自旋加合物均可被检测到。由于在呼吸爆发过程中也具有活性的髓过氧化物酶（MPO）在氯离子（Cl-）和过氧化氢（H2O2）存在的情况下会产生次氯酸（HOCl），因此该物质已被作为DMPO-OH加合物的可能来源进行了研究。在次氯酸浓度为0.1至0.7μmol/ml之间时，使用电子自旋共振（ESR）技术可检测到DMPO-OH自旋加合物。提出了形成该加合物的两种可能机制。这些发现表明，MPO的产物即次氯酸，是呼吸爆发过程中检测到的羟基自旋加合物的可能来源。
