a Eco-system Division, National Environmental Engineering Research Institute (NEERI) , Nagpur , India.
b School of Biotechnology , Devi Ahilya University , Indore , India.
Int J Phytoremediation. 2017 Dec 2;19(12):1085-1092. doi: 10.1080/15226514.2017.1328388.
A pot culture experiment was conducted for 90 days for the evaluation of oil and total petroleum hydrocarbon (TPH) degradation in vegetated and non-vegetated treatments of real-field oil-sludge-contaminated soil. Five different treatments include (T1) control, 2% oil-sludge-contaminated soil; (T2), augmentation of microbial consortium; (T3), Vertiveria zizanioides; (T4), bio-augmentation along with V. zizanioides; and (T5), bio-augmentation with V. zizanioides and bulking agent. During the study, oil reduction, TPH, and degradation of its fractions were determined. Physico-chemical and microbiological parameters of soil were also monitored simultaneously. At the end of the experimental period, oil content (85%) was reduced maximally in bio-augmented rhizospheric treatments (T4 and T5) as compared to control (27%). TPH reduction was observed to be 88 and 89% in bio-augmented rhizospheric soil (T4 and T5 treatments), whereas in non-rhizospheric and control (T2 and T1), TPH reduction was 78 and 37%, respectively. Degradation of aromatic fraction after 90 days in bio-augmented rhizosphere of treatments T4 and T5 was found to 91 and 92%, respectively. In microbial (T2) and Vertiveria treatments (T3), degradation of aromatic fraction was 83 and 68%, respectively. A threefold increase in soil dehydrogenase activity and noticeable changes in organic carbon content and water-holding capacity were also observed which indicated maximum degradation of oil and its fractions in combined treatment of plants and microbes. It is concluded that the plant-microbe soil system helps to restore soil quality and can be used as an effective tool for the remediation of oil-sludge-contaminated sites.
进行了为期 90 天的盆栽实验，以评估植被和非植被处理原状含油污泥污染土壤中石油和总石油烃（TPH）的降解情况。五个不同的处理包括：（T1）对照，2%含油污泥污染土壤；（T2）微生物联合体的添加；（T3）香根草；（T4）生物增强与香根草结合；（T5）生物增强与香根草和膨胀剂结合。在研究过程中，测定了石油减少量、TPH 及其各馏分的降解情况。同时监测了土壤的理化和微生物参数。在实验期末，与对照（27%）相比，生物增强根际处理（T4 和 T5）中油含量（85%）最大程度地降低。在生物增强根际土壤（T4 和 T5 处理）中观察到 TPH 降低了 88%和 89%，而在非根际和对照（T2 和 T1）中 TPH 降低了 78%和 37%。在生物增强根际处理 T4 和 T5 中，90 天后芳香馏分的降解率分别为 91%和 92%。在微生物（T2）和香根草（T3）处理中，芳香馏分的降解率分别为 83%和 68%。土壤脱氢酶活性增加了三倍，有机碳含量和持水能力也发生了明显变化，这表明植物和微生物联合处理能最大程度地降解石油及其各馏分。结论是植物-微生物-土壤系统有助于恢复土壤质量，可作为污染场地修复的有效工具。
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