Alsarraf Mohammed, Mohallal Eman M E, Mierzejewska Ewa J, Behnke-Borowczyk Jolanta, Welc-Falęciak Renata, Bednarska Małgorzata, Dziewit Lukasz, Zalat Samy, Gilbert Francis, Behnke Jerzy M, Bajer Anna
1 Department of Parasitology, Faculty of Biology, Institute of Zoology, University of Warsaw , Warsaw, Poland .
2 Desert Research Center , Cairo, Egypt .
Vector Borne Zoonotic Dis. 2017 Jul;17(7):483-494. doi: 10.1089/vbz.2016.2093. Epub 2017 May 25.
Bartonella spp. are parasites of mammalian erythrocytes and endothelial cells, transmitted by blood-feeding arthropod ectoparasites. Different species of rodents may constitute the main hosts of Bartonella, including several zoonotic species of Bartonella. The aim of this study was to identify and compare Bartonella species and genotypes isolated from rodent hosts from the South Sinai, Egypt. Prevalence of Bartonella infection was assessed in rodents (837 Acomys dimidiatus, 73 Acomys russatus, 111 Dipodillus dasyurus, and 65 Sekeetamys calurus) trapped in 2000, 2004, 2008, and 2012 in four dry montane wadis around St. Katherine town in the Sinai Mountains. Total DNA was extracted from blood samples, and PCR amplification and sequencing of the Bartonella-specific 860-bp gene fragment of rpoB and the 810-bp gene fragment of gltA were used for molecular and phylogenetic analyses. The overall prevalence of Bartonella in rodents was 7.2%. Prevalence differed between host species, being 30.6%, 10.8%, 9.6%, and 3.6% in D. dasyurus, S. calurus, A. russatus, and A. dimidiatus, respectively. The phylogenetic analyses of six samples of Bartonella (five from D. dasyurus and one from S. calurus) based on a fragment of the rpoB gene, revealed the existence of two distinct genetic groups (with 95-96% reciprocal sequence identity), clustering with several unidentified isolates obtained earlier from the same rodent species, and distant from species that have already been described (90-92% of sequence identity to the closest match from the GenBank reference database). Thus, molecular and phylogenetic analyses led to the description of two species: Candidatus Bartonella fadhilae n. sp. and Candidatus Bartonella sanaae n. sp. The identification of their vectors and the medical significance of these species need further investigation.
巴尔通体属是哺乳动物红细胞和内皮细胞的寄生虫，通过吸血节肢动物外寄生虫传播。不同种类的啮齿动物可能是巴尔通体的主要宿主，包括几种人兽共患病的巴尔通体种类。本研究的目的是鉴定和比较从埃及西奈半岛南部啮齿动物宿主中分离出的巴尔通体种类和基因型。评估了2000年、2004年、2008年和2012年在西奈山圣凯瑟琳镇周围四个干旱山地干谷捕获的啮齿动物（837只荒漠刺鼠、73只红刺鼠、111只长尾沙鼠和65只卡氏小鼠）中巴尔通体感染的患病率。从血液样本中提取总DNA，并使用巴尔通体特异性的rpoB基因860 bp片段和gltA基因810 bp片段的PCR扩增和测序进行分子和系统发育分析。啮齿动物中巴尔通体的总体患病率为7.2%。宿主物种之间的患病率有所不同，长尾沙鼠、卡氏小鼠、红刺鼠和荒漠刺鼠中的患病率分别为30.6%、10.8%、9.6%和3.6%。基于rpoB基因片段对六个巴尔通体样本（五个来自长尾沙鼠，一个来自卡氏小鼠）进行的系统发育分析显示，存在两个不同的遗传组（相互序列同一性为95 - 96%），与早期从相同啮齿动物物种获得的几个未鉴定分离株聚类，并且与已经描述的物种距离较远（与GenBank参考数据库中最接近匹配的序列同一性为90 - 92%）。因此，分子和系统发育分析导致了两个新物种的描述：新种暂定巴尔通体法迪拉菌和新种暂定巴尔通体萨纳菌。它们的传播媒介鉴定以及这些物种的医学意义需要进一步研究。
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