Liao Wanying, Fan Xinpeng, Zhang Qianqian, Xu Yuan, Gu Fukang
School of Life Sciences, East China Normal University, Shanghai, 200241, China.
Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China.
J Eukaryot Microbiol. 2018 Jan;65(1):48-60. doi: 10.1111/jeu.12431. Epub 2017 Jun 18.
The morphologies of two novel ciliates, Arcanisutura chongmingensis n. gen., n. sp. and Naxella paralucida n. sp., collected from Shanghai, China, have been investigated using live observation and silver staining methods. Arcanisutura n. gen. can be easily distinguished from related genera by its inconspicuous, oblique anterior suture. Arcanisutura chongmingensis n. sp. is mainly recognized by its elongated body with a tail-like posterior end, 25-33 somatic kineties, and 4-11 excretory pores. Naxella paralucida n. sp. can be distinguished from its congeners based on its two short nassulid organelles, fusiform trichocysts, 37-49 somatic kineties, and 16 nematodesmal rods. The small-subunit (SSU) rRNA gene sequences of these two species are presented, revealing the phylogenetic positions of Arcanisutura and Naxella. Phylogenetic analyses show that Arcanisutura forms a sister clade to other synhymeniid genera, namely, Chilodontopsis, Orthodonella, and Zosterodasys; Naxella is most closely related to Nassula spp. and is located within the monophyletic clade of the family Nassulidae.
利用活体观察和银染方法，对采自中国上海的两种新型纤毛虫——崇明隐秘缝毛虫（新属、新种）和类透明纳氏虫（新种）的形态进行了研究。隐秘缝毛虫新属可通过其不明显的斜向前缝合线与相关属轻易区分开来。崇明隐秘缝毛虫新种的主要特征为身体细长，后端呈尾状，有25 - 33条体动基列和4 - 11个排泄孔。类透明纳氏虫新种可根据其两个短小的鼻状细胞器、梭形刺丝泡、37 - 49条体动基列和16根线虫杆与其同属物种区分开来。给出了这两个物种的小亚基（SSU）rRNA基因序列，揭示了隐秘缝毛虫属和纳氏虫属的系统发育位置。系统发育分析表明，隐秘缝毛虫属与其他合膜目属，即 Chilodontopsis、Orthodonella 和 Zosterodasys 形成姐妹分支；纳氏虫属与 Nassula 属关系最为密切，位于 Nassulidae 科的单系分支内。
