Department of Orthopaedics, Biomechanics and Implant Technology Laboratory, University Medicine Rostock, Doberaner Strasse 142, Rostock, 18057, Germany.
J Biomed Mater Res A. 2017 Sep;105(9):2608-2615. doi: 10.1002/jbm.a.36114. Epub 2017 Jun 21.
Within the last ten years of biomedical implants, the focus is increasingly on bioceramics, specifically on zirconia (ZrO ). Hence, we analyzed the impact of ZrO particles in comparison to titanium particles on mature human osteoclasts (OCs) as little is known about the direct effect of wear particles on mature OCs and their role in the osteolytic process during aseptic endoprosthesis loosening. Changes in cell morphology and functionality of OCs incubated with particles in different concentrations were investigated in vitro. OCs tend to be enlarged after three days of cultivation with both types of particles, especially with high concentrations of ZrO , suggesting increased cell fusion. Further, we identified significantly increased expression of OC specific and bone matrix related genes: VNR, RANK, TRAP, and CTSK pointing on a direct stimulatory particle effect on the functionality of mature OCs. In completion, we quantified the bone resorption activity of particle treated mature OCs but could not detect a significant difference in bone resorption compared to OCs cultivated without particles. However, we could identify significantly higher gene expression of MMP-1 in particle treated OCs compared to untreated control OCs after three days of incubation. We also detected an impaired production of the tissue inhibitor of metalloproteinase, especially for OCs treated with high ZrO concentrations. In conclusion, our in vitro data show that abrasion particles could have a direct influence on mature OCs and therefore could promote increased OC-mediated bone resorption during aseptic loosening of total joint replacements. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2608-2615, 2017.
在过去十年的生物医学植入物中，重点越来越集中在生物陶瓷上，特别是氧化锆（ZrO ）。因此，我们分析了 ZrO 颗粒与钛颗粒对成熟人类破骨细胞（OC）的影响，因为关于磨损颗粒对成熟 OC 的直接影响及其在无菌假体松动过程中的溶骨性作用知之甚少。在体外研究了不同浓度颗粒孵育后 OC 细胞形态和功能的变化。两种类型的颗粒培养三天后，OC 倾向于增大，尤其是 ZrO 浓度较高时，表明细胞融合增加。此外，我们还发现 OC 特异性和骨基质相关基因的表达明显增加：VNR、RANK、TRAP 和 CTSK，这表明颗粒对成熟 OC 功能有直接刺激作用。此外，我们定量了颗粒处理成熟 OC 的骨吸收活性，但与未用颗粒培养的 OC 相比，未检测到骨吸收的显著差异。然而，我们可以发现，在培养三天后，与未经处理的对照 OC 相比，颗粒处理的 OC 中 MMP-1 的基因表达明显更高。我们还发现，组织金属蛋白酶抑制剂的产生受到了损害，尤其是对于高浓度 ZrO 处理的 OC。总之，我们的体外数据表明，磨损颗粒可能对成熟 OC 有直接影响，因此可能会促进全关节置换无菌松动期间 OC 介导的骨吸收增加。© 2017 Wiley Periodicals, Inc. J 生物材料 Res 部分 A：105A：2608-2615，2017。
