Human Nutrition Research Centre and Institute of Health and Society, Newcastle University, Newcastle upon Tyne, UK.
Human Nutrition Research Centre, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
Mol Nutr Food Res. 2017 Oct;61(10). doi: 10.1002/mnfr.201700142. Epub 2017 Jul 20.
Previous work highlighted the potential of odd-chain length saturated fatty acids as potential markers of dairy intake. The aim of this study was to assess the reproducibility of these biomarkers and their sensitivity to changes in dairy intake.
Fatty acid profiles and dietary intakes from food frequency questionnaires (FFQs) were measured three times over six months in the Food4Me Study. Reproducibility was explored through intra-class correlation coefficients (ICCs) and within-subject coefficients of variation (WCV). Sensitivity to changes in diet was examined using regression analysis. C15:0 blood levels showed high correlation over time (ICC: 0.62, 95% CI: 0.57, 0.68), however, the ICC for C17:0 was much lower (ICC: 0.32, 95% CI: 0.28, 0.46). The WCV for C15:0 was 16.6% and that for C17:0 was 14.6%. There were significant associations between changes in intakes of total dairy, high-fat dairy, cheese and butter and C15:0; and change in intakes of high-fat dairy and cream and C17:0.
Results provide evidence of reproducibility of C15:0 levels over time and sensitivity to change in intake of high-fat dairy products with results comparable to the well-established biomarker of fish intake (EPA+DHA).
先前的工作强调了奇数链长饱和脂肪酸作为乳制品摄入量潜在标志物的潜力。本研究旨在评估这些生物标志物的重现性及其对乳制品摄入量变化的敏感性。
在 Food4Me 研究中，在六个月内通过三次食物频率问卷（FFQ）测量脂肪酸谱和膳食摄入量。通过组内相关系数（ICC）和个体内变异系数（WCV）来探索重现性。使用回归分析检查对饮食变化的敏感性。C15:0 血水平随时间具有高度相关性（ICC：0.62，95%CI：0.57，0.68），然而，C17:0 的 ICC 低得多（ICC：0.32，95%CI：0.28，0.46）。C15:0 的 WCV 为 16.6%，C17:0 的 WCV 为 14.6%。总乳制品、高脂肪乳制品、奶酪和黄油摄入量的变化与 C15:0 之间存在显著关联；高脂肪乳制品和奶油摄入量的变化与 C17:0 之间存在显著关联。
结果提供了 C15:0 水平随时间重现性的证据，并且对高脂肪乳制品摄入量的变化敏感，结果与已确立的鱼类摄入量生物标志物（EPA+DHA）相当。
