Gollapudi S V, Vayuvegula B S, Thadepalli H, Gupta S
Department of Medicine, University of California, Irvine 92717.
J Clin Lab Immunol. 1988 Nov;27(3):121-5.
Ion channels play an important role in the transmission of signals across cell membrane in a variety of cell types. Recent studies indicate that normal murine splenic B lymphocytes express voltage gated K+ channels. In the present study, we examined the influence of K+ channel blockers 4 aminopyridine (4 AP) and tetraethyl ammonium, and Ca++ activated K+ channel blocker quinine on anti-immunoglobulin (anti-Ig)-induced B cell proliferation. The results show that ion channel blockers inhibited DNA synthesis in B cells induced by anti-Ig. 4 AP also inhibited protein synthesis but had no effect on the expression of surface immunoglobulin. 4 AP exerted its maximum suppressive effects on DNA synthesis when added within the first 24 hours after the onset of culture with anti-Ig. The inhibition of DNA synthesis by 4 AP was reversible upon its removal from culture. These studies show a role of K+ channels in B cell DNA and protein synthesis.
离子通道在多种细胞类型的细胞膜信号传递中发挥着重要作用。最近的研究表明，正常小鼠脾脏B淋巴细胞表达电压门控钾通道。在本研究中，我们检测了钾通道阻滞剂4-氨基吡啶（4-AP）和四乙铵，以及钙激活钾通道阻滞剂奎宁对抗免疫球蛋白（抗Ig）诱导的B细胞增殖的影响。结果表明，离子通道阻滞剂抑制了抗Ig诱导的B细胞中的DNA合成。4-AP还抑制了蛋白质合成，但对表面免疫球蛋白的表达没有影响。在与抗Ig一起培养开始后的头24小时内添加4-AP时，其对DNA合成发挥最大抑制作用。将4-AP从培养物中去除后，其对DNA合成的抑制作用是可逆的。这些研究表明钾通道在B细胞DNA和蛋白质合成中发挥作用。
