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营养载体吸附发酵提高了密粘褶菌漆酶产量。

Improved laccase production by Funalia trogii in absorbent fermentation with nutrient carrier.

作者信息

Li Guanhua, Liu Xiaoxue, Yuan Lin

机构信息

School of Life Sciences, Inner Mongolia University, Hohhot 010021, Inner Mongolia, People's Republic of China.

School of Life Sciences, Inner Mongolia University, Hohhot 010021, Inner Mongolia, People's Republic of China.

出版信息

J Biosci Bioeng. 2017 Oct;124(4):381-385. doi: 10.1016/j.jbiosc.2017.05.002. Epub 2017 May 22.

Abstract

A novel strategy of enhancing laccase production by absorbent fermentation was investigated. Peanut shell was used as nutrient carrier for laccase production by Funalia trogii IFP0027 in the absorbent fermentation. The maximum laccase production was reached to 11,900 U/l, which was 4.97 times higher than that of the control group. The results indicated that carbohydrates and phenolic substances especially flavonoids contained in peanut shell stimulated laccase production by F. trogii. Meanwhile, the peanut shell nutrient carrier could not only alleviate the oxidative damage, owing to strong scavenging activity on hydroxyl, but also relieve the mechanical stresses to form small and regular microbial pellets. Therefore, the absorbent fermentation using peanut shell as nutrient carrier shows enormous potential in enhancing laccase production.

摘要

研究了一种通过吸附发酵提高漆酶产量的新策略。在吸附发酵中,花生壳被用作营养载体,用于嗜热栖热菌IFP0027产漆酶。漆酶的最大产量达到11900 U/L,比对照组高4.97倍。结果表明,花生壳中含有的碳水化合物和酚类物质尤其是黄酮类化合物刺激了嗜热栖热菌产漆酶。同时,花生壳营养载体不仅可以减轻氧化损伤,因为其对羟基具有很强的清除活性,还可以缓解机械应力,形成小而规则的微生物颗粒。因此,以花生壳为营养载体的吸附发酵在提高漆酶产量方面具有巨大潜力。

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