Toth T E, Frank G H
Department of Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg 24061.
Am J Vet Res. 1988 Nov;49(11):1945-9.
Bovine parainfluenza-3 (BPI-3) virus proteins were radiolabeled with [35S]methionine. Control serum containing antibodies to BPI-3 virus obtained from calves that had been immunized by aerosol exposure and by IM inoculation and sera of cattle that were exposed to BPI-3 virus by aerosol only were used to immunoprecipitate BPI-3 virus proteins. The immunoprecipitates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Viral proteins with estimated molecular weights of 72,000, 70,000, and 58,000 were recognized by the control serum and the sera from calves exposed to BPI-3 virus only by aerosol. These proteins were the hemagglutinin/neuraminidase, nucleoprotein, and fusion protein. The results indicated that a single aerosol exposure to BPI-3 virus induces antibodies to the same BPI-3 virus proteins as do combined aerosol and parenteral inoculations with the virus.
牛副流感3型（BPI-3）病毒蛋白用[35S]甲硫氨酸进行放射性标记。从通过气溶胶暴露和肌肉注射免疫的犊牛获得的含有抗BPI-3病毒抗体的对照血清，以及仅通过气溶胶暴露于BPI-3病毒的牛血清，用于免疫沉淀BPI-3病毒蛋白。免疫沉淀物通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳进行分析。对照血清和仅通过气溶胶暴露于BPI-3病毒的犊牛血清识别出估计分子量为72,000、70,000和58,000的病毒蛋白。这些蛋白是血凝素/神经氨酸酶、核蛋白和融合蛋白。结果表明，单次气溶胶暴露于BPI-3病毒诱导产生的抗体与气溶胶和病毒的肠胃外接种联合诱导产生的抗体针对相同的BPI-3病毒蛋白。
