Department of Spinal Surgery, Nanfang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, Guangzhou, China.
Department of Spinal Surgery, LongYan First Hospital, Longyan, Fujian, China.
Calcif Tissue Int. 2017 Oct;101(4):412-421. doi: 10.1007/s00223-017-0292-1. Epub 2017 May 25.
To clarify osteoporotic effects of ketogenic diet (KD) on cancellous and cortical bone compared with ovariectomy (OVX) in mice. Forty female C57BL/6J 8-week-old mice were randomly divided into SD+Sham, SD+OVX, KD+Sham, and KD+OVX groups, and fed for 12 weeks. The distal femur of trabecular bone and the middle femur of cortical bone were evaluated with Micro-CT scanning. The maximum bending force and stiffness of the tibia were calculated using a three-point bending test. Osteoblast and osteoclast expression of femur were identified using tartrate-resistant acid phosphatase (TRAP), collagen type I (CoLI), and osteocalcin (OCN) staining. A 2-factor analysis of variance was used to evaluate effects of KD and OVX on radiological, biomechanical, and histological parameters. KD resulted in not only remarkable cancellous bone decline comparable to OVX, but also unique cortical bone reduction. The maximum bending force and stiffness decreased in the KD+Sham and KD+OVX groups but did not change in the SD+OVX group. The KD+OVX led to significantly higher expression in TRAP and noticeably lower expression in CoLI when compared with other groups. Both KD+Sham and SD+OVX prominently increased expression in TRAP, but decreased expression in CoLI. There was no significant difference in OCN among the four groups. The present results suggest that KD compromises both the cancellous and cortical bone architecture of long bones while OVX only in cancellous bone architecture. A combination of KD and OVX may lead to more bone loss.
为了阐明与卵巢切除术（OVX）相比，生酮饮食（KD）对小鼠松质骨和皮质骨的骨质疏松作用。将 40 只 8 周龄 C57BL/6J 雌性小鼠随机分为 SD+Sham、SD+OVX、KD+Sham 和 KD+OVX 组，并进行 12 周喂养。用 Micro-CT 扫描评估胫骨远端小梁骨和股骨中段皮质骨。用三点弯曲试验计算胫骨的最大弯曲力和刚度。通过抗酒石酸酸性磷酸酶（TRAP）、I 型胶原（CoLI）和骨钙素（OCN）染色鉴定股骨成骨细胞和破骨细胞的表达。采用双因素方差分析评估 KD 和 OVX 对影像学、生物力学和组织学参数的影响。KD 不仅导致明显的与 OVX 相当的松质骨下降，而且还导致独特的皮质骨减少。在 KD+Sham 和 KD+OVX 组中，最大弯曲力和刚度降低，但在 SD+OVX 组中没有改变。与其他组相比，KD+OVX 导致 TRAP 表达显著升高，CoLI 表达明显降低。四个组间 OCN 无显著差异。本研究结果表明，KD 会破坏长骨的松质骨和皮质骨结构，而 OVX 仅破坏松质骨结构。KD 和 OVX 的结合可能导致更多的骨丢失。
