Lankinen Åsa
Department of Theoretical Ecology, Lund University, Ecology Building, 22362, Lund, Sweden.
Oecologia. 2001 Aug;128(4):492-498. doi: 10.1007/s004420100681. Epub 2001 Aug 1.
In this study on Viola tricolor pollen, the competitive ability of 16 pollen donors originating from a wild population was analysed in a set of greenhouse and germination temperatures. The aim was to examine the consistency in donor pollen performance across temperatures and to see whether variation in performance was random or due to individual differences in the plastic response to temperature. Pollen tube growth rate in vitro was investigated in two greenhouse temperatures (on the day pollen was collected) and in four germination temperatures. In addition, pollen tube growth rate was assessed in vivo (in one temperature) to examine the relationship between in vivo and in vitro growth. A temperature difference of 5 K - corresponding to natural variation in time and space detected in the field - affected pollen tube growth rate. For both temperature components, significant pollen donor by temperature interactions were found and rank order of pollen donors changed across treatments. Although pollen competitive ability in violets was strongly influenced by both temperature components, the occurrence of pollen donor by temperature interactions indicates that donor siring ability varies with temperature. This, in turn, may suggest a means to maintain pollen competitive ability despite selection for this trait.
在这项关于三色堇花粉的研究中，分析了来自野生种群的16个花粉供体在一系列温室温度和萌发温度下的竞争能力。目的是检验不同温度下供体花粉表现的一致性，以及表现的差异是随机的，还是由于对温度的可塑性反应存在个体差异。在两个温室温度（花粉采集当天）和四个萌发温度下研究了花粉管的体外生长速率。此外，还在体内（在一个温度下）评估了花粉管的生长速率，以研究体内和体外生长之间的关系。5K的温差——相当于在田间检测到的时间和空间上的自然变化——影响了花粉管的生长速率。对于这两个温度因素，均发现了花粉供体与温度之间的显著交互作用，并且花粉供体的排名顺序在不同处理之间发生了变化。尽管紫罗兰的花粉竞争能力受到这两个温度因素的强烈影响，但花粉供体与温度之间的交互作用表明，供体的授精能力随温度而变化。这反过来可能暗示了一种在对该性状进行选择的情况下仍能维持花粉竞争能力的方法。
