Saito T
First Department of Oral Surgery, Hokkaido University School of Dentistry.
Hokkaido Igaku Zasshi. 1988 Nov;63(6):882-8.
We investigated the intracellular cyclic AMP (cAMP) level and cytotoxic sensitivity to anti-tumor-associated antigen (anti-TAA) antibody in transplantable rat fibrosarcoma cells with regard to each of three days following ip transplantation. The cytotoxic sensitivity of the 3-methylcholanthrene (MCA)-induced transplantable fibrosarcoma KMT-17 cells to anti-TAA antibody decreased daily after ip transplantation in syngeneic WKA/Hok rats. In contrast, the intracellular cAMP level of the tumor cells increased daily after ip transplantation, and the cAMP level of 3-day-old KMT-17 tumor cells (1.31 pmol/10(6) cells) was statistically (p less than 0.01) higher than that of 1-day-old tumor cells (1.01 pmol/10(6) cells). This phenomenon disappeared, however, after artificial infection of the tumor cells with Friend murine leukemia virus (F-MuLV). The cytotoxic sensitivity of F-MuLV-infected KMT-17 (FV-KMT-17) tumor cells to anti-TAA antibody did not decrease and no elevation of the intracellular cAMP level was observed (0.92-0.97 pmol/10(6) cells), regardless of the number of days after ip transplantation. The cytotoxic sensitivity of KMT-17 and FV-KMT-17 tumor cells to anti-TAA antibody was therefore in inverse proportion to the intracellular cAMP levels of the tumor cells.(ABSTRACT TRUNCATED AT 250 WORDS)
我们研究了可移植性大鼠纤维肉瘤细胞在腹腔注射移植后三天内，细胞内环磷酸腺苷（cAMP）水平以及对抗肿瘤相关抗原（抗TAA）抗体的细胞毒性敏感性。在同基因WKA/Hok大鼠中，腹腔注射移植后，3-甲基胆蒽（MCA）诱导的可移植性纤维肉瘤KMT-17细胞对抗TAA抗体的细胞毒性敏感性每日下降。相反，移植后肿瘤细胞的细胞内cAMP水平每日升高，3日龄KMT-17肿瘤细胞的cAMP水平（1.31 pmol/10(6) 个细胞）在统计学上（p<0.01）高于1日龄肿瘤细胞（1.01 pmol/10(6) 个细胞）。然而，在用Friend小鼠白血病病毒（F-MuLV）人工感染肿瘤细胞后，这种现象消失了。无论腹腔注射移植后的天数如何，F-MuLV感染的KMT-17（FV-KMT-17）肿瘤细胞对抗TAA抗体的细胞毒性敏感性均未降低，且未观察到细胞内cAMP水平升高（0.92 - 0.97 pmol/10(6) 个细胞）。因此，KMT-17和FV-KMT-17肿瘤细胞对抗TAA抗体的细胞毒性敏感性与肿瘤细胞的细胞内cAMP水平成反比。（摘要截短于250字）
