von Gersdorff Jørgensen L, Kania P W, Rasmussen K J, Mattsson A H, Schmidt J, Al-Jubury A, Sander A, Salanti A, Buchmann K
Laboratory of Aquatic Pathobiology at the Department of Veterinary and Animal Sciences, University of Copenhagen, Frederiksberg, Denmark.
Department of Cancer and Inflammation Research, University of Southern Denmark, Odense, Denmark.
J Fish Dis. 2017 Dec;40(12):1815-1821. doi: 10.1111/jfd.12653. Epub 2017 May 26.
The protective effect in rainbow trout (Oncorhynchus mykiss) of an experimental subunit vaccine targeting antigens in the parasite Ichthyophthirius multifiliis has been evaluated and compared to effects elicited by a classical parasite homogenate vaccine. Three recombinant parasite proteins (two produced in E. coli and one in insect cells) were combined and injected i.p., and subsequently, protection and antibody responses were analysed. Both the experimental and the benchmark vaccine induced partial but significant protection against I. multifiliis when compared to control fish. Specific antibody responses of vaccinated trout (subunit vaccine) were raised against one neurohypophysial n-terminal domain protein #10 of three recombinant proteins, whereas the benchmark vaccine group showed specific antibody production against all three recombinant proteins. The immunogenic parasite protein #10 may be a potential vaccine candidate supplementing the protective I-antigen in future vaccine trials.
已对一种针对寄生虫多子小瓜虫（Ichthyophthirius multifiliis）抗原的实验性亚单位疫苗对虹鳟（Oncorhynchus mykiss）的保护效果进行了评估，并与传统寄生虫匀浆疫苗所引发的效果进行了比较。将三种重组寄生虫蛋白（两种在大肠杆菌中产生，一种在昆虫细胞中产生）混合后腹腔注射，随后分析保护效果和抗体反应。与对照鱼相比，实验疫苗和基准疫苗均诱导了对多子小瓜虫的部分但显著的保护作用。接种疫苗的鳟鱼（亚单位疫苗）针对三种重组蛋白中的一种神经垂体n端结构域蛋白#10产生了特异性抗体反应，而基准疫苗组针对所有三种重组蛋白均产生了特异性抗体。具有免疫原性的寄生虫蛋白#10可能是一种潜在的疫苗候选物，可在未来的疫苗试验中补充保护性I抗原。
