Côrte-Real S, Grimaldi Juńior G, De Meirelles M de N
Instituto Oswaldo Cruz, Departamento de Ultraestrutura e Biologia Celular, Rio de Janeiro, Brasil.
Mem Inst Oswaldo Cruz. 1988 Jan-Mar;83(1):113-21. doi: 10.1590/s0074-02761988000100015.
The degree of maturation of cells of the Mononuclear Phagocyte System (MPS), during in vivo and in vitro infection by Leishmania mexicana amazonensis, was evaluated in this study. The macrophages' differentiation was assayed by cytochemical characterization at the ultrastructural level, using two well-established markers: 5'-nucleotidase enzyme activity, for revealing the mature cells; and the peroxidase activity present in the cell granules to demonstrate immature mononuclear phagocytes. Only a few macrophages, demonstrating 5'-nucleotidase positive reaction in both the plasma membrane and within their cytoplasmic vesicles, were found scattered in the chronic inflammation at the L. m. amazonensis lesions in albino mice. However, by the peroxidase activity analysis, we were also able to demonstrate the presence of immature MPS cells, which predominate, together with parasitized vacuolated macrophages, in chronic lesions induced in this system by L. m. amazonensis. The implications of these results on the pathogenesis of murine cutaneous leishmaniasis are discussed.
在本研究中，评估了亚马逊利什曼原虫在体内和体外感染期间，单核吞噬细胞系统（MPS）细胞的成熟程度。巨噬细胞的分化通过超微结构水平的细胞化学特征进行分析，使用两种成熟的标志物：5'-核苷酸酶活性，用于揭示成熟细胞；以及细胞颗粒中存在的过氧化物酶活性，以证明未成熟的单核吞噬细胞。在白化小鼠亚马逊利什曼原虫病变处的慢性炎症中，仅发现少数巨噬细胞在其质膜和细胞质小泡内均表现出5'-核苷酸酶阳性反应。然而，通过过氧化物酶活性分析，我们还能够证明未成熟MPS细胞的存在，在该系统中由亚马逊利什曼原虫诱导的慢性病变中，未成熟MPS细胞与寄生的空泡化巨噬细胞一起占主导地位。讨论了这些结果对鼠类皮肤利什曼病发病机制的影响。
