Mashkovskaia G V, Denisova T S, Lovanok E V, Fomicheva V V, Kartel' N A
Mol Gen Mikrobiol Virusol. 1988 Dec(12):11-6.
The study of the plasmid R388 deletional derivatives has shown the antitumorigenicity determinant of the plasmid to be contained by the tra-operon. Transposon Tn5 has been used for selection of a number of insertional mutants having lost the antitumorigenic properties. The difference in the locations of Tn5 inserts was found, all of the latter being localized within the tra-operon. The data obtained suggest the locus of the plasmid R388 responsible for its antitumorigenic properties to be located in the region of tra-genes distally to the rep-region.
对质粒R388缺失衍生物的研究表明，该质粒的抗肿瘤决定簇由转移操纵子所包含。转座子Tn5已被用于筛选许多失去抗肿瘤特性的插入突变体。发现了Tn5插入位置的差异，所有这些插入都位于转移操纵子内。所获得的数据表明，质粒R388中负责其抗肿瘤特性的基因座位于转移基因区域中远离复制区域的远端。
