Canaz Emel, Kilinc Metin, Sayar Hamide, Kiran Gurkan, Ozyurek Eser
Gynecologic Oncology, Department of Obstetrics and Gynecology, Sisli Hamidiye Etfal Training and Research Hospital, Halaskargazi Cd. Etfal, Sisli, Sk. 34371 Turkey.
Department of Biochemistry, Kahramanmaras Sutcu Imam University School of Medicine, Kahramanmaras, Turkey.
J Trace Elem Med Biol. 2017 Sep;43:217-223. doi: 10.1016/j.jtemb.2017.05.003. Epub 2017 May 17.
Wide variation exists in ovarian cancer incidence rates suggesting the importance of environmental factors. Due to increasing environmental pollution, trace elements and heavy metals have drawn attention in studies defining the etiology of cancer, but scant data is available for ovarian cancer. Our aim was to compare the tissue concentrations of lead, selenium and nickel in epithelial ovarian cancer, borderline tumor and healthy ovarian tissues.
The levels of lead, selenium and nickel were estimated using atomic absorption spectrophotometry in formalin-fixed paraffin-embedded tissue samples. Tests were carried out in 20 malignant epithelial ovarian cancer, 15 epithelial borderline tumor and 20 non-neoplastic healthy ovaries. Two samples were collected for borderline tumors, one from papillary projection and one from the smooth surface of cyst wall.
Pb and Ni concentrations were found to be higher both in malignant and borderline tissues than those in healthy ovaries. Concentrations of Pb and Ni in malignant tissues, borderline papillary projections and capsular tissue samples were not different. Comparison of Se concentrations of malignant, borderline and healthy ovarian tissues did not reveal statistical difference. Studied metal levels were not found to be different in either papillary projection or in cyst wall of the borderline tumors.
This study revealed the accumulation of lead and nickel in ovarian tissue is associated with borderline and malignant proliferation of the surface epithelium. Accumulation of these metals in epithelial ovarian cancer and borderline ovarian tumor has not been demonstrated before.
卵巢癌发病率存在广泛差异，提示环境因素的重要性。由于环境污染日益严重，微量元素和重金属在癌症病因学研究中受到关注，但关于卵巢癌的数据却很少。我们的目的是比较上皮性卵巢癌、交界性肿瘤和健康卵巢组织中铅、硒和镍的组织浓度。
采用原子吸收分光光度法测定福尔马林固定石蜡包埋组织样本中铅、硒和镍的含量。对20例恶性上皮性卵巢癌、15例上皮性交界性肿瘤和20例非肿瘤性健康卵巢进行检测。交界性肿瘤采集两个样本，一个来自乳头状突起，一个来自囊壁光滑表面。
发现恶性组织和交界性组织中的铅和镍浓度均高于健康卵巢。恶性组织、交界性乳头状突起和包膜组织样本中的铅和镍浓度无差异。恶性、交界性和健康卵巢组织中硒浓度的比较未显示出统计学差异。在交界性肿瘤的乳头状突起或囊壁中，所研究的金属水平没有差异。
本研究表明卵巢组织中铅和镍的积累与表面上皮的交界性和恶性增殖有关。这些金属在上皮性卵巢癌和交界性卵巢肿瘤中的积累此前尚未得到证实。
