Zhang Yun, Yan Chenghui, Yang Hang, Yu Junping, Wei Hongping
Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei 430071, PR China; University of Chinese Academy of Sciences, Beijing 100039, PR China; Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine, School of Laboratory Medicine, Xinxiang Medical University, Xinxiang, Henan 453003, PR China.
Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei 430071, PR China; University of Chinese Academy of Sciences, Beijing 100039, PR China.
Food Chem. 2017 Nov 1;234:332-338. doi: 10.1016/j.foodchem.2017.05.013. Epub 2017 May 4.
Mammal IgG antibodies are normally used in conventional immunoassays for E. coli O157:H7, which could lead to false positive results from the presence of protein A producing S. aureus. In this study, a natural specific bacteriophage was isolated and then conjugated with magnetic beads as a capture element in a sandwich format for the rapid and selective detection of E. coli O157:H7. To the best of our knowledge, it was the first time to utilize a natural bacteriophage to develop a phagomagnetic separation combined with colorimetric assay for E. coli O157:H7. The method has an overall time less than 2h with a detection limit of 4.9×10CFU/mL. No interference from S. aureus was observed. Furthermore, the proposed method was successfully applied to detect E. coli O157:H7 in spiked skim milk. The proposed detection system provided a potential method for E. coli O157:H7 and other pathogenic bacteria in food samples.
哺乳动物IgG抗体通常用于大肠杆菌O157:H7的传统免疫测定中，而产蛋白A的金黄色葡萄球菌的存在可能导致假阳性结果。在本研究中，分离出一种天然特异性噬菌体，然后将其与磁珠偶联，作为夹心形式的捕获元件，用于快速、选择性检测大肠杆菌O157:H7。据我们所知，这是首次利用天然噬菌体开发用于大肠杆菌O157:H7的噬菌磁分离结合比色测定法。该方法总时长不到2小时，检测限为4.9×10CFU/mL。未观察到金黄色葡萄球菌的干扰。此外，所提出的方法成功应用于检测加标的脱脂乳中的大肠杆菌O157:H7。所提出的检测系统为食品样品中的大肠杆菌O157:H7和其他病原菌提供了一种潜在的检测方法。
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