Balejčíková L, Garamus V M, Avdeev M V, Petrenko V I, Almásy L, Kopčanský P
Institute of Experimental Physics, Slovak Academy of Sciences, Watsonova 47, 04001 Košice, Slovakia.
Helmholtz-Zentrum Geesthacht, Centre for Materials and Coastal Research, Max-Planck-Street 1, 21502 Geesthacht, Germany.
Colloids Surf B Biointerfaces. 2017 Aug 1;156:375-381. doi: 10.1016/j.colsurfb.2017.05.036. Epub 2017 May 18.
The structural stability of magnetoferritin, a synthetic analogue of ferritin, at various pH levels is assessed here. The structural and electrical properties of the complexes were determined by small-angle X-ray scattering (SAXS), dynamic light scattering (DLS) and zeta potential measurements. At pH 3-6 a reduction of electrostatic repulsion on the suspended colloids resulted in aggregation and sedimentation of magnetoferritin. At neutral to slightly alkaline conditions (pH 7-9) the magnetoferritin structure was stable for lower iron loadings. Higher solution pH 10-12 induced destabilization of the protein structure and dissociation of subunits. Increasing the loading factor in the MFer complex leads to decrease of the stability versus pH changes.
本文评估了铁蛋白的合成类似物磁铁蛋白在不同pH值水平下的结构稳定性。通过小角X射线散射（SAXS）、动态光散射（DLS）和zeta电位测量来确定复合物的结构和电学性质。在pH 3 - 6时，悬浮胶体上静电排斥力的降低导致磁铁蛋白聚集和沉淀。在中性至微碱性条件（pH 7 - 9）下，较低铁负载量时磁铁蛋白结构稳定。较高的溶液pH值10 - 12会导致蛋白质结构不稳定和亚基解离。增加MFer复合物中的负载因子会导致其稳定性随pH变化而降低。
