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vPARP与多药耐药蛋白共同调节耐药细胞系中MVP的表达。

vPARP Adjusts MVP Expression in Drug-resistant Cell Lines in Conjunction with MDR Proteins.

作者信息

Wojtowicz Karolina, Januchowski Radoslaw, Nowicki Michal, Zabel Maciej

机构信息

Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland

Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland.

出版信息

Anticancer Res. 2017 Jun;37(6):3015-3023. doi: 10.21873/anticanres.11656.

DOI:10.21873/anticanres.11656

PMID:28551640

Abstract

BACKGROUND/AIM: The definition of vault (ribonucleoprotein particles) function remains highly complex. Vaults may cooperate with multidrug resistance (MDR) proteins, supporting their role in drug resistance. This topic is the main theme of this publication.

MATERIALS AND METHODS

The cell viability was determined by an MTT assay. The protein expression was detected by western blot analysis. The proteins were knocked-down using siRNA.

RESULTS

No major vault protein (MVP) in the LoVo/Dx and W1PR cell lines after tunicamycin treatment was shown. In W1PR cells with knocked-down MVP, a statistically significant decrease in cell viability was noted. In LoVo/Dx, W1TR and A2780TR cells were vault poly-ADP-ribose polymerase (vPARP) was knockdown, a decrease in cell viability was shown. Also, MVP silencing induced an increase in glycoprotein P (Pgp) expression in LoVo/Dx cells.

CONCLUSION

MVP is important for the drug resistance of cancer cells, but it probably requires the presence of vPARP for full activation. Some correlations between MDR proteins and vaults exist.

摘要

背景/目的：穹窿体（核糖核蛋白颗粒）功能的定义仍然极为复杂。穹窿体可能与多药耐药（MDR）蛋白协同作用，支持它们在耐药性方面的作用。本主题是本出版物的主要内容。

材料与方法

通过MTT法测定细胞活力。通过蛋白质印迹分析检测蛋白质表达。使用小干扰RNA（siRNA）敲低蛋白质。

结果

衣霉素处理后，LoVo/Dx和W1PR细胞系中未显示主要穹窿体蛋白（MVP）。在MVP敲低的W1PR细胞中，观察到细胞活力有统计学意义的下降。在LoVo/Dx、W1TR和A2780TR细胞中穹窿体聚ADP核糖聚合酶（vPARP）被敲低后，显示细胞活力下降。此外，MVP沉默诱导LoVo/Dx细胞中糖蛋白P（Pgp）表达增加。