Heinonen P K, Punnonen R, Teisala K, Ashorn R, Kujansuu E, Seppälä E, Mörsky P, Metsä-Ketelä T
Department of Obstetrics and Gynaecology, Tampere University Central Hospital, Finland.
Acta Eur Fertil. 1988 Nov-Dec;19(6):325-8.
The concentrations of cyclic AMP, cyclic GMP, prostaglandin E2 (PGE2), leukotriene B4 (LTB4) oestradiol, progesterone and luteinizing hormone (LH) were determined in 30 follicular fluid samples from 25 women in an IVF programme. Multiple follicular development was induced by clomiphene citrate and human menopausal gonadotropin. Laparoscopic aspiration of follicular fluid was performed 36 hours after injection of human chorionic gonadotropin. The mean concentration of LH was significantly lower (p less than 0.05) in the follicular fluid with ova developing to embryos that cleaved than in those without cleavage. The mean concentrations of cAMP, cGMP, PGE2, LTB4, oestradiol and progesterone were not significantly different. PGE2 and LH concentrations in the follicular fluids showed significant correlation (r = 0.56; p = 0.004). Significant correlation was also seen between cAMP and progesterone concentrations (r = 0.50; p = 0.006). The presence of cyclic nucleotides, PGE2 and LTB4 in human follicular fluid suggests that these factors may have a role in the function of the follicular fluid.
对25名接受体外受精（IVF）程序的女性的30份卵泡液样本进行了环磷酸腺苷（cAMP）、环磷酸鸟苷（cGMP）、前列腺素E2（PGE2）、白三烯B4（LTB4）、雌二醇、孕酮和促黄体生成素（LH）浓度的测定。通过枸橼酸氯米芬和人绝经期促性腺激素诱导多个卵泡发育。在注射人绒毛膜促性腺激素36小时后，通过腹腔镜抽吸卵泡液。与未发生卵裂的卵泡相比，卵发育为发生卵裂的胚胎的卵泡液中LH的平均浓度显著降低（p<0.05）。cAMP、cGMP、PGE2、LTB4、雌二醇和孕酮的平均浓度无显著差异。卵泡液中PGE2和LH浓度呈显著相关（r = 0.56；p = 0.004）。cAMP和孕酮浓度之间也存在显著相关（r = 0.50；p = 0.006）。人卵泡液中环核苷酸、PGE2和LTB4的存在表明这些因素可能在卵泡液的功能中发挥作用。
