Araya-Hidalgo Edward, Gutiérrez-Jiménez Cristina, Chaves-Ramírez Mónica, Suárez-Esquivel Marcela, Guzmán-Verri Caterina, Barquero-Calvo Elías
Programa de Investigación en Enfermedades Tropicales, Escuela de Medicina Veterinaria, Universidad Nacional, Heredia, Costa Rica.
Physiology Department, Escuela de Medicina Veterinaria, Universidad Nacional, Heredia, Costa Rica.
J Vet Med Sci. 2017 Jul 19;79(7):1210-1214. doi: 10.1292/jvms.16-0530. Epub 2017 May 27.
The hmtp210 gene of Avibacterium paragallinarum, the causative agent of infectious coryza, encodes an outer-membrane hemagglutinin (HA) that plays an essential role in pathogenicity. A hypervariable region within this HA, which is highly antigenic, is proposed as a candidate for recombinant vaccine production. Nonetheless, little is known about its genetic variability. We performed sequencing analysis of the hmtp210 hypervariable region in 16 clinical isolates from Costa Rica and compared them with 4 vaccine strains and the hmtp210 sequences available in public databases. Except for isolate ApCR12, all isolates showed high identity with reference vaccine strains 0083 and H18. Better genetic characterization of the hypervariable region of hmtp210 is necessary to develop better immunogenic strategies and improved molecular typing methods.
鸡传染性鼻炎病原体副鸡禽杆菌的hmtp210基因编码一种外膜血凝素（HA），该血凝素在致病性中起关键作用。该HA内的一个高变区具有高度抗原性，被提议作为重组疫苗生产的候选区域。然而，对其遗传变异性知之甚少。我们对来自哥斯达黎加的16株临床分离株的hmtp210高变区进行了测序分析，并将它们与4株疫苗株以及公共数据库中可用的hmtp210序列进行了比较。除分离株ApCR12外，所有分离株与参考疫苗株0083和H18均显示出高度同源性。为了制定更好的免疫策略和改进分子分型方法，有必要对hmtp210高变区进行更好的遗传特征分析。
