有氧运动联合虎纹毒素-I减轻慢性脑缺血损伤。

Aerobic exercise combined with huwentoxin-I mitigates chronic cerebral ischemia injury.

作者信息

Mao Hai-Feng, Xie Jun, Chen Jia-Qin, Tang Chang-Fa, Chen Wei, Zhou Bo-Cun, Chen Rui, Qu Hong-Lin, Wu Chu-Zu

机构信息

Key Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, Hunan Normal University, Changsha, Hunan Province, China.

College of Physical Education, Yichun University, Yichun, Jiangxi Province, China.

出版信息

Neural Regen Res. 2017 Apr;12(4):596-602. doi: 10.4103/1673-5374.205099.

Ca channel blockers have been shown to protect neurons from ischemia, and aerobic exercise has significant protective effects on a variety of chronic diseases. The present study injected huwentoxin-I (HWTX-I), a spider peptide toxin that blocks Ca channels, into the caudal vein of a chronic cerebral ischemia mouse model, once every 2 days, for a total of 15 injections. During this time, a subgroup of mice was subjected to treadmill exercise for 5 weeks. Results showed amelioration of cortical injury and improved neurological function in mice with chronic cerebral ischemia in the HWTX-I + aerobic exercise group. The combined effects of HWTX-I and exercise were superior to HWTX-I or aerobic exercise alone. HWTX-I effectively activated the Notch signal transduction pathway in brain tissue. Aerobic exercise up-regulated synaptophysin mRNA expression. These results demonstrated that aerobic exercise, in combination with HWTX-I, effectively relieved neuronal injury induced by chronic cerebral ischemia the Notch signaling pathway and promoting synaptic regeneration.

钙通道阻滞剂已被证明可保护神经元免受缺血损伤，有氧运动对多种慢性疾病具有显著的保护作用。本研究将一种阻断钙通道的蜘蛛肽毒素胡文毒素-I（HWTX-I）注入慢性脑缺血小鼠模型的尾静脉，每2天注射一次，共注射15次。在此期间，将一组小鼠进行为期5周的跑步机运动。结果显示，HWTX-I+有氧运动组慢性脑缺血小鼠的皮质损伤得到改善，神经功能得到改善。HWTX-I和运动的联合作用优于单独使用HWTX-I或有氧运动。HWTX-I有效激活脑组织中的Notch信号转导通路。有氧运动上调了突触素mRNA的表达。这些结果表明，有氧运动与HWTX-I联合使用可有效减轻慢性脑缺血诱导的神经元损伤，通过Notch信号通路促进突触再生。