Ozkisacik Sezen, Erdem Ali Onur, Etensel Barlas, Tataroglu Canten, Serter Mukadder, Yazici Mesut
1 Department of Pediatric Surgery, Faculty of Medicine, Adnan Menderes University, Aydin, Turkey.
2 Department of Pathology, Faculty of Medicine, Adnan Menderes University, Aydin, Turkey.
J Int Med Res. 2017 Jun;45(3):1036-1041. doi: 10.1177/0300060517708921. Epub 2017 May 28.
Objective Acute mesenteric ischaemia leads to intestinal damage. Restoration of blood flow results in further damage to tissue, which is called reperfusion injury. This study aimed to investigate the protective effects of short-interval postconditioning and to determine the optimal interval for reperfusion in an experimental rat model of intestinal ischaemia. Methods Forty adult male Wistar rats were grouped as follows: sham (Sh), ischaemia + reperfusion (IR), ischaemia + postconditioning for 5 seconds (PC5), ischaemia + postconditioning for 10 seconds (PC10), and ischaemia + postconditioning for 20 seconds (PC20). For postconditioning, 10 cycles of reperfusion (5, 10, or 20 seconds) interspersed by 10 cycles of 10 seconds of ischaemia were performed. Blood glutathione reductase (GR) and glutathione peroxidase (GPx) levels were measured. Intestinal tissue damage was assessed histopathologically. Results GR levels were significantly higher in the PC5 group than in the IR group (37.7 ± 9.0 vs. 18.5 ± 2.0 min/g Hb). GPx levels were significantly higher in the PC10 group than in the IR group (43.2 ± 9.2 vs. 15.9 ± 4.6 U/g Hb). The histopathological score was significantly lower in the PC5 group (1.1 ± 0.1) than in the IR group (2.1 ± 0.2). Conclusion Short-interval postconditioning reduces reperfusion injury in the ischaemic bowel and the optimal interval for reperfusion is 5 seconds. The long-term effects of short-interval postconditioning and the optimal reperfusion interval in intestinal ischaemia-reperfusion in rats need to be investigated.
目的 急性肠系膜缺血会导致肠道损伤。血流恢复会导致组织进一步损伤，这被称为再灌注损伤。本研究旨在探讨短时间间隔后处理的保护作用，并确定在大鼠肠道缺血实验模型中的最佳再灌注间隔。方法 将40只成年雄性Wistar大鼠分为以下几组：假手术组（Sh）、缺血+再灌注组（IR）、缺血+5秒后处理组（PC5）、缺血+10秒后处理组（PC10）和缺血+20秒后处理组（PC20）。对于后处理，进行10个周期的再灌注（5、10或20秒），其间穿插10个周期的10秒缺血。测量血液中谷胱甘肽还原酶（GR）和谷胱甘肽过氧化物酶（GPx）水平。通过组织病理学评估肠道组织损伤。结果 PC5组的GR水平显著高于IR组（37.7±9.0对18.5±2.0分钟/克血红蛋白）。PC10组的GPx水平显著高于IR组（43.2±9.2对15.9±4.6单位/克血红蛋白）。PC5组的组织病理学评分（1.1±0.1）显著低于IR组（2.1±0.2）。结论 短时间间隔后处理可减轻缺血肠段的再灌注损伤，最佳再灌注间隔为5秒。短时间间隔后处理的长期影响以及大鼠肠道缺血再灌注中的最佳再灌注间隔仍需进一步研究。
