Muller H M, Schuber F
Laboratoire de Chimie Enzymatique, CNRS UA 1182, Institut de Botanique, Strasbourg, France.
Biochem Int. 1988 Nov;17(5):953-63.
NAD+ glycohydrolase, an amphipathic membrane-bound enzyme, solubilized from calf spleen microsomes with detergents and purified, was immobilized by hydrophobic interactions on octyl-Sepharose. Conditions are described for optimal adsorption on the gel. The immobilized enzyme remained catalytically active (hydrolase and transglycosidase activities) and could be used to prepare nicotinic acid analogs of NAD(P)+.
NAD⁺ 糖水解酶是一种两亲性膜结合酶，用去污剂从小牛脾脏微粒体中溶解并纯化后，通过疏水相互作用固定在辛基琼脂糖上。文中描述了在凝胶上进行最佳吸附的条件。固定化酶保持催化活性（水解酶和转糖苷酶活性），可用于制备 NAD(P)⁺ 的烟酸类似物。
