Properties of native calf spleen NAD+ glycohydrolase immobilized by hydrophobic interaction.

NAD+ glycohydrolase, an amphipathic membrane-bound enzyme, solubilized from calf spleen microsomes with detergents and purified, was immobilized by hydrophobic interactions on octyl-Sepharose. Conditions are described for optimal adsorption on the gel. The immobilized enzyme remained catalytically active (hydrolase and transglycosidase activities) and could be used to prepare nicotinic acid analogs of NAD(P)+.