肌联蛋白/连接蛋白的电泳研究细微差别
Nuances of electrophoresis study of titin/connectin.
作者信息
Vikhlyantsev Ivan M, Podlubnaya Zoya A
机构信息
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya Street 3, Pushchino, 142290, Russia.
Pushchino State Institute of Natural Science, Nauki Street 3, Pushchino, 142290, Russia.
出版信息
Biophys Rev. 2017 Jun;9(3):189-199. doi: 10.1007/s12551-017-0266-6. Epub 2017 May 29.
Abstract
Almost 40 years has passed since the discovery of giant elastic protein titin (also known as connectin) of striated and smooth muscles using gel electrophoresis. Sodium dodecyl sulfate polyacrylamide gel electrophoresis is a major technique for studying the isoform composition and content of titin. This review provides historical insights into the technical aspects of the electrophoresis methods used to identify titin and its isoforms. We particularly focus on the nuances of the technique that improve the preservation of its primary structure so that its high molecular weight isoforms can be visualized.
摘要
自利用凝胶电泳发现横纹肌和平滑肌中的巨大弹性蛋白肌联蛋白（也称为伴肌动蛋白）以来，已经过去了近40年。十二烷基硫酸钠聚丙烯酰胺凝胶电泳是研究肌联蛋白同工型组成和含量的主要技术。本综述提供了对用于鉴定肌联蛋白及其同工型的电泳方法技术方面的历史见解。我们特别关注该技术的细微差别，这些差别有助于更好地保留其一级结构，从而能够观察到其高分子量同工型。
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