Department of Toxicology and Biometrology, National Institute for Research and Safety (INRS), 54019, Vandoeuvre cedex, France.
Contact Dermatitis. 2017 Nov;77(5):311-322. doi: 10.1111/cod.12829. Epub 2017 May 29.
Identification of the allergenic potency of chemicals is a key step in the safety assessment process. Predictive assays that require no or few animals are needed.
To develop an alternative in vitro mouse bone marrow-derived dendritic cell (BMDC) assay to determine the allergenic potential of chemicals.
BMDCs were exposed to well-known allergens and to non-allergenic chemicals. Surface marker expression and cytokine release of BMDCs were analysed after treatment.
Eleven tested chemicals showed a significant stimulation index (SI) of >1.5 (accuracy, 75%; sensitivity, 69%). The four non-allergens all showed a SI of <1.5. Eight contact allergens tested showed a significant SI of >1.5 (accuracy, 92%; sensitivity, 89%), whereas only two respiratory allergens showed a significant SI of >1.5 (accuracy, 60%; sensitivity, 33%).
The results indicate that the BMDC assay could become a reliable test for assessment of the allergenic potential of chemicals. The next step should include the testing of further chemicals, with the aim of integrating this assay into the toolbox of in vitro methods for the evaluation of the allergenic potential of chemicals.
鉴定化学物质的致敏效力是安全性评估过程中的关键步骤。需要开发无需或只需少量动物的预测性检测方法。
开发替代的体外小鼠骨髓来源树突状细胞（BMDC）检测方法，以确定化学物质的致敏潜力。
将 BMDC 暴露于已知过敏原和非致敏化学物质中。处理后分析 BMDC 的表面标记物表达和细胞因子释放。
11 种测试化学物质的刺激指数（SI）>1.5（准确性为 75%；灵敏度为 69%）。四种非变应原的 SI 均<1.5。测试的 8 种接触变应原的 SI>1.5（准确性为 92%；灵敏度为 89%），而仅有 2 种呼吸变应原的 SI>1.5（准确性为 60%；灵敏度为 33%）。
结果表明，BMDC 检测方法可成为评估化学物质致敏潜力的可靠检测方法。下一步应包括进一步测试其他化学物质，目的是将该检测方法整合到用于评估化学物质致敏潜力的体外方法工具包中。
