Laboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, Shenyang, PR China.
J Sep Sci. 2017 Jul;40(14):3008-3014. doi: 10.1002/jssc.201700051. Epub 2017 Jun 20.
A rapid and cost-effective method based on microwave-assisted extraction followed by capillary electrophoresis was developed for simultaneous quantification of seven alkaloids in Corydalis decumbens for the first time. The main parameters affecting microwave-assisted extraction and capillary electrophoresis separation were investigated and optimized. The optimal microwave-assisted extraction was performed at 40°C for 5 min using methanol/water (90:10, v/v) as the extracting solvent. Electrophoretic separation was achieved within 15 min using an uncoated fused-silica capillary (50 μm internal diameter and 27.7 cm effective length) and a 500 mM Tris buffer containing 45% v/v methanol (titrated to pH 2.86 with H PO ). The developed method was successfully applied to the quantification of seven alkaloids in Corydalis decumbens obtained from different regions of China. The combination of microwave-assisted extraction with capillary electrophoresis was an effective method for the rapid analysis of the alkaloids in Corydalis decumbens.
首次建立了一种基于微波辅助提取结合毛细管电泳的快速、经济的方法，用于同时定量测定伏毛铁棒锤中的 7 种生物碱。考察并优化了影响微波辅助提取和毛细管电泳分离的主要参数。最佳的微波辅助提取条件为：甲醇/水（90:10，v/v）为提取溶剂，40°C 提取 5 min。采用未涂层的熔融石英毛细管（50 µm 内径，27.7 cm 有效长度），在 500 mM Tris 缓冲液中含 45%v/v 甲醇（用 H₃PO₄ 调至 pH 2.86），可在 15 min 内实现电泳分离。该方法成功应用于测定来自中国不同地区的伏毛铁棒锤中 7 种生物碱的含量。微波辅助提取与毛细管电泳相结合是一种快速分析伏毛铁棒锤中生物碱的有效方法。
