高效液相色谱法测定伏生紫堇中四种生物碱的含量

[Determination of four alkaloids in Corydalis decumbens by HPLC].

作者信息

Shen Yan, Han Chao, Xia Biqi, Zhou Yongfang, Liu Cuiping, Liu Aili

机构信息

College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou 325035, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2011 Aug;36(15):2110-2.

PMID:22066451

Abstract

OBJECTIVE

To establish a quantitative HPLC method for determination of protopine, palmatine hydrochloride, bicuculline and tetrahydropalmatine, in Corydalis decumbens.

METHOD

The separation was performed on a ZORBAX Eclipse XDB-C18 column (4.6 mm x 150 mm, 5 microm) at a flow rate of 1.0 mL x min(-1) using mixtures of two solvents [A(20 mmol x L(-1) ammonium acetate)-B(acetonitrile)]: with a gradient elution. The column oven temperature was 30 degrees C and the detection wavelength was set at 280 nm.

RESULT

The 4 alkaloids were well separated by this HPLC method. Linearifies of protopine, palmatine hydrochloride, bicuculline and tetrahydropalmatine were good in the ranges of 1.44-46.0 (r = 0.999 4), 1.2640.2 (r = 0.999 8), 1.37-44.0 (r = 0.999 9), and 1.3643.6 mg x L(-1) (r = 0.999 9), respectively. The average recoveries were 98.2% with RSD 2.7% for protopine, 101.9% with RSD 2.5% for palmatine hydrochloride, 102.8% with RSD 3.5% for tetrahydropalmatine, and 98.8% with RSD 3.1% for tetrahydropalmatine.

CONCLUSION

This method is proved to be convenient, reliable and accurate., and it can be used for quality control of C. decumbens.

摘要

目的

建立一种高效液相色谱法定量测定伏生紫堇中原阿片碱、盐酸巴马汀、荷包牡丹碱和延胡索乙素的含量。

方法

采用ZORBAX Eclipse XDB-C18柱（4.6 mm×150 mm，5 µm），以两种溶剂[A（20 mmol·L⁻¹醋酸铵）-B（乙腈）]的混合物为流动相，流速为1.0 mL·min⁻¹，进行梯度洗脱。柱温为30℃，检测波长设定为280 nm。

结果

该高效液相色谱法能很好地分离这4种生物碱。原阿片碱、盐酸巴马汀、荷包牡丹碱和延胡索乙素的线性关系良好，线性范围分别为1.44 - 46.0（r = 0.999 4）、1.26 - 40.2（r = 0.999 8）、1.37 - 44.0（r = 0.999 9）和1.36 - 43.6 mg·L⁻¹（r = 0.999 9）。原阿片碱的平均回收率为98.2%，相对标准偏差为2.7%；盐酸巴马汀的平均回收率为101.9%，相对标准偏差为2.5%；延胡索乙素的平均回收率为102.8%，相对标准偏差为3.5%；荷包牡丹碱的平均回收率为98.8%，相对标准偏差为3.1%。