Kou Tian-Chao, Liu Yue-Tong, Li Ming, Yang Yang, Zhang Wei, Cui Ji-Hui, Zhang Xue-Wen, Dong Shu-Mei, Xu Sha, You Shan, Yu Dong-Shuai, Pang Zun-Yu, Luo Kai-Jun
School of Life Sciences, Yunnan University, Kunming, P.R. China and Key Laboratory for Animal Genetic Diversity and Evolution of High Education in Yunnan Province, Yunnan University, Kunming, P.R. China.
Arch Insect Biochem Physiol. 2017 Jun;95(2). doi: 10.1002/arch.21389. Epub 2017 May 30.
Two physiological changes of Spodoptera litura parasitized by Microplitis bicoloratus are hemocyte-apoptosis and retarded immature development. β-Chain of F F -ATPase was found from a S. litura transcriptome. It belongs to a conserved P-loop NTPase superfamily, descending from a common ancestor of Lepidopteran clade. However, the characterization of β-chain of ATPase in apoptotic cells and its involvement in development remain unknown. Here, the ectopic expression and endogenous F F -ATPase β-chain occurred on S. litura cell membrane: in vivo, at the late stage of apoptotic hemocyte, endogenous F F -ATPase β-chain was stably expressed during M. bicoloratus larva development from 4 to 7 days post-parasitization; in vitro, at an early stage of pre-apoptotic Spli221 cells by infecting with M. bicoloratus bracovirus particles, the proteins were speedily recover expression. Furthermore, endogenous F F -ATPase β-chain was localized on the apoptotic cell membrane. RNA interference (RNAi) of F F -ATPase β-chain led to significantly decreased head capsule width. This suggested that F F -ATPase β-chain positively regulated the development of S. litura. The RNAi effect on the head capsule width was enhanced with parasitism. Our research found that F F -ATPase β-chain was expressed and localized on the cell membrane in the apoptotic cells, and involved in the development of S. litura.
被双色绿茧蜂寄生的斜纹夜蛾有两种生理变化，即血细胞凋亡和未成熟发育迟缓。从斜纹夜蛾转录组中发现了F₀F₁ -ATP酶的β链。它属于保守的P环NTP酶超家族，起源于鳞翅目进化枝的一个共同祖先。然而，凋亡细胞中ATP酶β链的特性及其在发育中的作用仍不清楚。在此，异位表达和内源性F₀F₁ -ATP酶β链出现在斜纹夜蛾细胞膜上：在体内，在凋亡血细胞的后期，内源性F₀F₁ -ATP酶β链在双色绿茧蜂幼虫寄生后4至7天的发育过程中稳定表达；在体外，在用双色绿茧蜂杆状病毒颗粒感染处于凋亡前期的Spli221细胞的早期，这些蛋白质迅速恢复表达。此外，内源性F₀F₁ -ATP酶β链定位于凋亡细胞膜上。对F₀F₁ -ATP酶β链进行RNA干扰（RNAi）导致头壳宽度显著减小。这表明F₀F₁ -ATP酶β链对斜纹夜蛾的发育起正向调节作用。寄生会增强RNAi对头壳宽度的影响。我们的研究发现，F₀F₁ -ATP酶β链在凋亡细胞的细胞膜上表达并定位，并参与斜纹夜蛾的发育。
