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前列腺素E2在体外通过EP2和EP4促进人CD34+细胞归巢。

Prostaglandin E2 promotes human CD34+ cells homing through EP2 and EP4 in vitro.

作者信息

Wang Yaqun, Lai Shuping, Tang Jing, Feng Chun, Liu Fangjie, Su Chang, Zou Waiyi, Chen Huizhen, Xu Duorong

机构信息

Department of Hematology, The First Affiliated Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China.

出版信息

Mol Med Rep. 2017 Jul;16(1):639-646. doi: 10.3892/mmr.2017.6649. Epub 2017 May 30.

DOI:10.3892/mmr.2017.6649
PMID:28560401
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5482140/
Abstract

Recently, certain studies have demonstrated in vitro that prostaglandin E2 (PGE2) promotes human cluster of differentiation (CD)34+ cell homing. However, the sub‑type receptors activated by PGE2 are unknown, as the PGE2 receptor EP1-4 subtypes (EP1-4) are expressed on the membrane of human CD34+ cells. Based on the above, the present study aimed to screen the receptor subtype activity by PGE2 to promote human CD34+ cell homing. It was observed that human CD34+ cells expressed the four PGE2 sub‑receptors, particularly EP2 and 4. PGE2 increased EP2 and 4 mRNA expression significantly, while EP1 and 3 mRNA exhibited no significant alteration. PGE2, EP2 agonist (EP2A), and EP4A upregulated C‑X‑C chemokine receptor 4 mRNA and protein expression in human CD34+ cells, and promoted stromal cell‑derived factor 1α (SDF‑1α) expression in bone marrow mesenchymal stem cells (BMMSCs). These phenomena were inhibited by the associated receptor antagonists. PGE2, EP2A, and EP4A facilitated human CD34+ cell migration towards SDF‑1α and BMMSCs. The results of the present study suggested that PGE2 promoted human CD34+ cell homing through EP2 and 4 receptors in vitro.

摘要

最近，某些研究已在体外证明前列腺素E2（PGE2）可促进人分化簇（CD）34+细胞归巢。然而，PGE2激活的亚型受体尚不清楚，因为PGE2受体EP1-4亚型（EP1-4）在人CD34+细胞膜上表达。基于上述情况，本研究旨在筛选PGE2促进人CD34+细胞归巢的受体亚型活性。观察到人CD34+细胞表达四种PGE2亚型受体，尤其是EP2和EP4。PGE2显著增加EP2和EP4的mRNA表达，而EP1和EP3的mRNA无明显变化。PGE2、EP2激动剂（EP2A）和EP4激动剂（EP4A）上调人CD34+细胞中C-X-C趋化因子受体4的mRNA和蛋白表达，并促进骨髓间充质干细胞（BMMSC）中基质细胞衍生因子1α（SDF-1α）的表达。这些现象被相关受体拮抗剂抑制。PGE2、EP2A和EP4A促进人CD34+细胞向SDF-1α和BMMSC迁移。本研究结果表明，PGE2在体外通过EP2和EP4受体促进人CD34+细胞归巢。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b97e/5482140/711948c28831/MMR-16-01-0639-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b97e/5482140/8fb20177b1e3/MMR-16-01-0639-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b97e/5482140/ba13d5c5bc47/MMR-16-01-0639-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b97e/5482140/df8572ac629a/MMR-16-01-0639-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b97e/5482140/bada29951be3/MMR-16-01-0639-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b97e/5482140/2a99b4e09539/MMR-16-01-0639-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b97e/5482140/711948c28831/MMR-16-01-0639-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b97e/5482140/8fb20177b1e3/MMR-16-01-0639-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b97e/5482140/ba13d5c5bc47/MMR-16-01-0639-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b97e/5482140/df8572ac629a/MMR-16-01-0639-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b97e/5482140/bada29951be3/MMR-16-01-0639-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b97e/5482140/2a99b4e09539/MMR-16-01-0639-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b97e/5482140/711948c28831/MMR-16-01-0639-g05.jpg

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