Wang Haizhen, Wu Xiangwei
Department of Clinical Cancer Prevention, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX, 77030, USA.
Adv Exp Med Biol. 2017;994:133-141. doi: 10.1007/978-3-319-55947-6_7.
Circulating tumor cells (CTCs) disseminate from solid primary cancers into the peripheral blood and lymphatic vessels and can lead to metastatic tumor development; thus, CTC assays are an important clinical tool for monitoring progression and evaluating prognosis in cancer. However, CTCs are limited in number and heterogeneous in their biological and physical properties, making their detection, isolation, and enumeration a major challenge. To overcome these difficulties, novel techniques have been developed to detect and enumerate CTCs with an invasive phenotype. In this chapter, we will summarize these recently developed methods and detail two novel methods for capturing and enriching CTCs on the basis of their viability and their invasive properties.
循环肿瘤细胞（CTCs）从实体原发性癌症扩散到外周血和淋巴管中，并可导致转移性肿瘤的发展；因此，CTCs检测是监测癌症进展和评估预后的重要临床工具。然而，CTCs数量有限，其生物学和物理特性存在异质性，这使得它们的检测、分离和计数成为一项重大挑战。为了克服这些困难，已开发出新技术来检测和计数具有侵袭性表型的CTCs。在本章中，我们将总结这些最近开发的方法，并详细介绍两种基于CTCs的活力和侵袭特性来捕获和富集CTCs的新方法。
