Food Chemistry and Technology Department Teagasc Food Research Centre, Moorepark, Fermoy, Ireland
Department of Life Sciences, University of Limerick, Limerick, Ireland
Curr Issues Mol Biol. 2017;23:21-38. doi: 10.21775/cimb.023.021. Epub 2017 May 31.
Outbreaks of infections have emphasized the necessity for rapid and economic detection methods for pathogens in samples ranging from those of clinical origin to food products during production and retail storage, and increasingly, in environmental samples. Flow cytometry (FCM) allows the rapid acquisition of multi-parametric data regarding cell populations within fluidised samples. However, the application of FCM to pathogen detection depends on the availability of specific fluorescent probes such as antibodies and RNA probes capable of detecting and isolating pathogens from these diverse samples. A particular issue for FCM methodology is the ability to recover and discriminate bacteria from the sample matrix which may pose a major technical hurdle towards accurate and sensitive analysis. This review article focuses on detection of pathogens using FCM in samples originating from food, water, environmental and clinical sources and outlines the current state of the art and potential future applications.
感染爆发强调了在从临床来源到生产和零售储存过程中的食品产品，以及越来越多的环境样本中，对病原体进行快速和经济的检测方法的必要性。流式细胞术（FCM）允许快速获取关于流体化样品中细胞群体的多参数数据。然而，FCM 在病原体检测中的应用取决于是否有特定的荧光探针，如能够从这些不同的样品中检测和分离病原体的抗体和 RNA 探针。FCM 方法学的一个特别问题是从可能对准确和敏感分析构成重大技术障碍的样品基质中回收和区分细菌的能力。本文综述了使用源自食品、水、环境和临床来源的样品的 FCM 检测病原体的方法，并概述了当前的技术现状和潜在的未来应用。
