Resolution of soluble rat cardiac phosphodiesterases by high performance liquid chromatography.
作者信息
Bode D C, Kanter J R, Brunton L L
机构信息
Department of Pharmacology, University of California, San Diego, La Jolla.
出版信息
Second Messengers Phosphoproteins. 1988;12(5-6):235-40.
PMID:2856111
Abstract
A high performance liquid chromatographic method has been developed to separate isozymes of cyclic nucleotide phosphodiesterase. The method employs a polymer-based anion exchange column eluted with a sodium chloride gradient. Compared to traditional chromatography over DEAE-cellulose, the method is more rapid (30 min), dilutes the sample less, achieves better resolution of kinetically distinct forms, may be applied to as little as 200 micrograms of tissue protein and is appropriate for analytical use.
摘要
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