Simmet T, Luck W
Department of Pharmacology and Toxicology, Ruhr-University Bochum, Federal Republic of Germany.
Eicosanoids. 1988;1(2):107-10.
A crude membrane fraction from guinea-pig splenic tissue containing leukotriene (LT) B4-binding sites was prepared and used in a radioreceptor assay for the quantitative determination of LTB4 in clotting whole human blood as well as in eluates from reversed-phase HPLC. Binding of [3H]LTB4 to the binding sites was inhibited by 50% with 0.31 ng LTB4. The relative cross-reaction of 20-OH-LTB4, 20-COOH-LTB4 and 12-hydroxyeicosatetraenoic acid (HETE) was 9.51%, 0.41% and 0.03%, respectively. The relative cross-reaction of various other prostanoids, cysteinyl-LT, mono-HETE's and arachidonic acid was below 0.01%. The spleen binding sites are useful for a rapid, simple and specific quantification of LTB4 in biological material.
制备了来自豚鼠脾脏组织的含有白三烯（LT）B4结合位点的粗膜级分，并将其用于放射受体分析，以定量测定凝血全血以及反相高效液相色谱洗脱液中的LTB4。0.31 ng LTB4可使[3H]LTB4与结合位点的结合被抑制50%。20-羟基-LTB4、20-羧基-LTB4和12-羟基二十碳四烯酸（HETE）的相对交叉反应分别为9.51%、0.41%和0.03%。其他各种前列腺素、半胱氨酰白三烯、单羟基二十碳四烯酸和花生四烯酸的相对交叉反应低于0.01%。脾脏结合位点可用于快速、简单且特异性地定量生物材料中的LTB4。
