一种用于钙调蛋白测定的分光光度法。
A spectrophotometric method for calmodulin assay.
作者信息
Garg U C, Rai N, Singh Y, Dhaunsi G S, Sidhu G S, Ganguly N K, Bhatnagar R
机构信息
Post Graduate Institute of Medical Education and Research, Chandigarh, India.
出版信息
Biotechniques. 1988 Apr;6(4):294-6.
PMID:2856235
Abstract
The activity of calmodulin as an activator of cAMP phosphodiesterase was assayed. AMP was hydrolyzed by 5'-nucleotidase, and the adenosine formed was measured by both liquid scintillation counting and spectrophotometry at 265 nm. Calmodulin activities measured by the two methods were equivalent, indicating that spectrophotometric assay of calmodulin can be used in place of the isotopic method.
摘要
对作为环磷酸腺苷磷酸二酯酶激活剂的钙调蛋白活性进行了测定。5'-核苷酸酶可水解AMP，通过液体闪烁计数法和在265nm处的分光光度法来测定生成的腺苷。用这两种方法测得的钙调蛋白活性相当，这表明钙调蛋白的分光光度法测定可替代同位素法。
相似文献
1
A spectrophotometric method for calmodulin assay.一种用于钙调蛋白测定的分光光度法。
Biotechniques. 1988 Apr;6(4):294-6.
2
An optimized continuous assay for cAMP phosphodiesterase and calmodulin.
Anal Biochem. 1984 Apr;138(1):34-43. doi: 10.1016/0003-2697(84)90765-6.
3
A new method for measurement of cyclic AMP phosphodiesterase activity.
J Biochem. 1977 Aug;82(2):387-94.
4
Molecular interaction between cAMP and calcium in calmodulin-dependent cyclic nucleotide phosphodiesterase system.钙调蛋白依赖性环核苷酸磷酸二酯酶系统中cAMP与钙之间的分子相互作用。
Clin Invest Med. 1994 Aug;17(4):374-82.
5
PDE4: arrested at the border.磷酸二酯酶4：在边界处受阻。
Sci STKE. 2003 Oct 14;2003(204):PE44. doi: 10.1126/stke.2003.204.pe44.
6
Cyclic AMP turnover in intact tissue. Role of cyclic nucleotide phosphodiesterases.完整组织中的环磷酸腺苷周转。环核苷酸磷酸二酯酶的作用。
Adv Second Messenger Phosphoprotein Res. 1992;25:1-11.
7
A rapid and precise assay for calmodulin and cyclic AMP phosphodiesterase on a centrifugal analyser.
Clin Chim Acta. 1987 May 15;164(3):305-13. doi: 10.1016/0009-8981(87)90305-6.
8
Phosphodiesterase type 4 expression and anti-proliferative effects in human pulmonary artery smooth muscle cells.人肺动脉平滑肌细胞中4型磷酸二酯酶的表达及抗增殖作用。
Respir Res. 2006 Jan 19;7(1):9. doi: 10.1186/1465-9921-7-9.
9
7-Bromo-1,5-dihydro-3,6-dimethylimidazo[2,1-b]quinazolin-2(3H)- one (Ro 15-2041), a potent antithrombotic agent that selectively inhibits platelet cyclic AMP-phosphodiesterase.7-溴-1,5-二氢-3,6-二甲基咪唑并[2,1-b]喹唑啉-2(3H)-酮（Ro 15-2041），一种选择性抑制血小板环磷酸腺苷磷酸二酯酶的强效抗血栓形成剂。
J Pharmacol Exp Ther. 1985 Oct;235(1):212-9.
10
Cyclic-3',5'-nucleotide phosphodiesterases in the cyclic adenosine monophosphate (cAMP)-mediated actions of vasopressin.
Semin Nephrol. 1994 Jul;14(4):333-40.
引用本文的文献
1
Ca(2+)-Mg (2+)-dependent ATP-ase activity in hemodialyzed children. Effect of a hemodialysis session.血液透析儿童中钙（Ca2+）-镁（Mg2+）依赖性 ATP 酶活性。血液透析治疗的影响。
Pediatr Nephrol. 2010 Dec;25(12):2501-7. doi: 10.1007/s00467-010-1634-7. Epub 2010 Sep 30.
Zotero 插件