Ballmer-Hofer K, Ziegler A, Burger M M
Friedrich Miescher-Institut, Basel, Switzerland.
Oncogene. 1988 Oct;3(4):365-71.
Polyoma virus middle T antigen (mT) as well as pp60v-src, the oncogene product of Rous sarcoma virus, associate with membranes and cytoskeletal structures in virus-infected cells. Furthermore, mT has been shown to be tightly bound to pp60c-src, the cellular homolog of pp60v-src. While the presence of pp60v-src in focal contacts has been demonstrated, the localization of mT and pp60c-src is less clear. We show here that mT is associated with focal contacts and that the phosphorylated protein can be immunoprecipitated with anti-vinculin serum. We suggest that the mT/pp60c-src complex and pp60v-src are tightly bound to vinculin. This interaction may be relevant in the process of cell transformation since disorganization of the actin filament network seems to play an important role in the deregulation of cell growth observed in tumor cells.
多瘤病毒中间T抗原（mT）以及劳氏肉瘤病毒的癌基因产物pp60v-src，在病毒感染的细胞中与细胞膜和细胞骨架结构相关联。此外，mT已被证明与pp60v-src的细胞同源物pp60c-src紧密结合。虽然已经证实pp60v-src存在于粘着斑中，但mT和pp60c-src的定位尚不清楚。我们在此表明，mT与粘着斑相关联，并且磷酸化蛋白可以用抗纽蛋白血清进行免疫沉淀。我们认为mT/pp60c-src复合物和pp60v-src与纽蛋白紧密结合。这种相互作用可能在细胞转化过程中具有相关性，因为肌动蛋白丝网络的紊乱似乎在肿瘤细胞中观察到的细胞生长失调中起重要作用。
