Gaillot D, Terver D, Finance C, Schwartzbrod L
Faculté de Pharmacie, Nancy, France.
Int J Food Microbiol. 1988 Jun;6(4):333-9. doi: 10.1016/0168-1605(88)90027-x.
An experimental method for contaminating mussels with poliovirus that approaches natural conditions in a coastal zone is described. A 280 liter aquarium is used to determine optimum conditions for obtaining a maximum titer of virus in mussels. When mussels were kept for 1 h in water contaminated with from 3 to 368 PFU ml-1, between 1 to 628 PFU g-1 tissue were recovered. Mussels exposed to contaminated water for periods of up to 10 days showed decreased titers of virus which seemed to parallel the natural inactivation of viruses in water with time. 1 h of exposure to contaminated water was always sufficient for a maximum adsorption of viral particles by mussels.
本文描述了一种使贻贝受到脊髓灰质炎病毒污染的实验方法，该方法接近沿海地区的自然条件。使用一个280升的水族箱来确定在贻贝中获得最高病毒滴度的最佳条件。当贻贝在含有3至368 PFU/ml脊髓灰质炎病毒的污染水中放置1小时后，每克组织中可回收1至628 PFU的病毒。暴露于污染水中长达10天的贻贝，其病毒滴度降低，这似乎与水中病毒随时间的自然失活情况相似。贻贝暴露于污染水中1小时，总能使其最大限度地吸附病毒颗粒。
