使用马来酰亚胺或高碘酸盐制备的Fab'-过氧化物酶缀合物对线粒体蛋白硫辛酰胺脱氢酶进行免疫组织化学染色的比较。
Comparison of immunohistochemical staining of a mitochondrial protein, lipoamide dehydrogenase, with Fab'-peroxidase conjugates prepared by maleimide or periodate.
作者信息
Matuda S, Yagi Y, Saheki T, Hashida S, Ishikawa E
机构信息
Department of Biochemistry, School of Medicine, Kagoshima University, Japan.
出版信息
In Vivo. 1987 Jul-Aug;1(4):221-7.
IgG-maleimide peroxidase, Fab'-maleimide peroxidase, polymer and monomer types of Fab'-periodate peroxidase were prepared from an antibody against rat lipoamide dehydrogenase, a component of the pyruvate dehydrogenase complex which is located in mitochondria. They were examined for immunohistochemical staining of the rat kidney. Fab'-maleimide peroxidase was the best for staining mitochondrial protein. IgG-maleimide peroxidase and the monomer type of Fab'-periodate peroxidase had the same intensity of staining. The polymer type of Fab'-periodate peroxidase could not stain the lipoamide dehydrogenase.
IgG-马来酰亚胺过氧化物酶、Fab'-马来酰亚胺过氧化物酶、聚合物型和单体型Fab'-高碘酸盐过氧化物酶是由抗大鼠硫辛酰胺脱氢酶的抗体制备而成，硫辛酰胺脱氢酶是位于线粒体中的丙酮酸脱氢酶复合体的一个组成部分。对它们进行了大鼠肾脏的免疫组织化学染色检测。Fab'-马来酰亚胺过氧化物酶对线粒体蛋白染色效果最佳。IgG-马来酰亚胺过氧化物酶和单体型Fab'-高碘酸盐过氧化物酶染色强度相同。聚合物型Fab'-高碘酸盐过氧化物酶无法对硫辛酰胺脱氢酶进行染色。
Zotero 插件