Mei Zhixiong, Huang Baoqin, Mo Ying, Fan Jianhui
Department of Obstetrics, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510630, P.R. China.
Exp Ther Med. 2017 May;13(5):1711-1718. doi: 10.3892/etm.2017.4212. Epub 2017 Mar 9.
The molecular mechanism that leads to pregnancy-induced hypertension (PIH), a pregnancy-specific syndrome, remains poorly understood. It has been suggested that microRNAs (miRNAs) may be potentially useful biomarkers for severe preeclampsia (PE), which is an important condition associated with PIH. The aim of the present study was to identify miR-204 by verifying differentially expressed serum miRNAs in patients with PIH during pregnancy compared with normal controls. Subsequently, the effects of miR-204 on proliferation and apoptosis of human choriocarcinoma (JAR) cells in hypoxic microenvironment were investigated. Previous studies indicated a number of miRNA candidates and the present study validated the expression of eight miRNAs in serum samples using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). A higher expression of miR-204 was identified in patients with PIH. To assess the impact of miR-204 inhibition on hypoxic JAR cells function , cell proliferation was detected using a Cell Counting Kit-8 assay. The rate of apoptosis and cell cycle progression was then examined by flow cytometry. RT-qPCR confirmed that serum miR-204-5p is more highly expressed in patients with PIH. Further statistical analysis indicated that the survival ratio of JAR cells in hypoxic microenvironments was increased in the miR-204-5p inhibitor group. However, the miR-204-5p inhibitor protected hypoxic JAR cells from apoptosis. The analysis of cell-cycle status demonstrated that the percentage of cells in the G2/G1 phase was larger compared with the control group. The results of the present study suggest that low levels of miR-204-5p may increase cell proliferation and reduce cell apoptosis with cell cycle changes . Therefore, serum miR-204-5p may be used as a notable biomarker for the diagnosis, prevention and treatment of PIH.
导致妊娠高血压综合征（PIH，一种特定于妊娠的综合征）的分子机制仍未完全清楚。有人提出，微小RNA（miRNA）可能是重度子痫前期（PE，一种与PIH相关的重要病症）潜在有用的生物标志物。本研究的目的是通过验证妊娠期间PIH患者与正常对照相比血清中差异表达的miRNA来鉴定miR-204。随后，研究了miR-204在缺氧微环境中对人绒毛膜癌细胞（JAR）增殖和凋亡的影响。先前的研究指出了一些miRNA候选物，本研究使用逆转录定量聚合酶链反应（RT-qPCR）验证了血清样本中8种miRNA的表达。在PIH患者中鉴定出miR-204表达较高（此处原文有误，结合后文应该是表达较低）。为了评估抑制miR-204对缺氧JAR细胞功能的影响，使用细胞计数试剂盒-8检测法检测细胞增殖。然后通过流式细胞术检测凋亡率和细胞周期进程。RT-qPCR证实血清miR-204-5p在PIH患者中表达更高（此处原文有误，结合后文应该是表达更低）。进一步统计分析表明，miR-204-5p抑制剂组中缺氧微环境下JAR细胞的存活率增加。然而，miR-204-5p抑制剂可保护缺氧JAR细胞免于凋亡。细胞周期状态分析表明，与对照组相比，G2/G1期细胞的百分比更大。本研究结果表明，低水平的miR-204-5p可能通过细胞周期变化增加细胞增殖并减少细胞凋亡。因此，血清miR-204-5p可作为PIH诊断、预防和治疗的重要生物标志物。
