McCarty R, Plunkett L M
Department of Psychology, University of Virginia, Charlottesville 22903.
Peptides. 1988;9 Suppl 1:3-8. doi: 10.1016/0196-9781(88)90215-x.
In two experiments, binding sites for atrial natriuretic factor (ANF) were studied in discrete areas of rat brain by quantitative autoradiography. In the first experiment, the maximum binding capacity of 125I-ANF was reduced significantly in the subfornical organ and choroid plexus of 4 and 14 week old spontaneously hypertensive (SHR) rats compared to aged-matched Wistar-Kyoto (WKY) normotensive controls. In contrast, the maximum binding capacity of 125I-ANF in the area postrema was similar for young and adult SHR and WKY rats. The second experiment involved a comparison of brain ANF binding sites in Long-Evans control rats and Brattleboro rats with inherited diabetes insipidus. The maximum binding capacity of 125I-ANF was significantly greater in the subfornical organ of Brattleboro rats compared to Long-Evans controls. However, no strain differences occurred for 125I-ANF binding in the choroid plexus or area postrema. These findings indicate that the number of ANF binding sites in discrete areas of rat brain may be influenced in a highly selective fashion by alterations in body fluid homeostasis (i.e., hypertension or diabetes insipidus). Changes in brain ANF binding sites within circumventricular areas may involve central as well as peripheral sources of ANF-related peptides.
在两项实验中，通过定量放射自显影术研究了大鼠脑离散区域中心房钠尿肽（ANF）的结合位点。在第一项实验中，与年龄匹配的Wistar-Kyoto（WKY）正常血压对照相比，4周龄和14周龄自发性高血压（SHR）大鼠的穹窿下器官和脉络丛中125I-ANF的最大结合能力显著降低。相反，成年和幼年SHR大鼠及WKY大鼠最后区中125I-ANF的最大结合能力相似。第二项实验比较了Long-Evans对照大鼠和遗传性尿崩症的Brattleboro大鼠脑中ANF结合位点。与Long-Evans对照相比，Brattleboro大鼠穹窿下器官中125I-ANF的最大结合能力显著更高。然而，脉络丛或最后区中125I-ANF结合不存在品系差异。这些发现表明，大鼠脑离散区域中ANF结合位点的数量可能受到体液稳态改变（即高血压或尿崩症）的高度选择性影响。脑室周区域内脑ANF结合位点的变化可能涉及ANF相关肽的中枢和外周来源。
