Chandra Rampalli Viswa, Sailaja Sistla, Reddy Aileni Amarender
Department of Periodontics, SVS Institute of Dental Sciences, Mahabubnagar, Telangana, India.
Gerodontology. 2017 Sep;34(3):382-389. doi: 10.1111/ger.12279. Epub 2017 Jun 1.
The aim of this study was to estimate tissue and gingival crevicular fluid (GCF) levels of the oxidative stress marker 8-hydroxy-2'-deoxyguanosine (8-OHdG) in premenopausal, perimenopausal and postmenopausal women with chronic periodontitis.
Oxidative stress has been implicated in the etiopathogenesis of periodontitis and menopause induces oxidative stress.
According to Stages of Reproductive Aging Workshop (STRAW) criteria, women diagnosed with periodontitis were subdivided into three groups of 31 participants each 1. Premenopausal 2. Perimenopausal and 3. Postmenopausal. GCF and gingival tissue samples were collected from sites with maximum probing depth. Tissue DNA was extracted from the gingival sample and 8-OHdG in the extracted DNA, and GCF samples were measured using ELISA.
There was a highly significant difference in the overall GCF 8-OHdG levels among the three groups with the pairwise difference being highly significant between the premenopausal-postmenopausal groups and perimenopausal-postmenopausal groups. However, no overall significant differences in tissue 8-OHdG levels were found among the three groups. Pairwise, highly significant differences were found between the premenopausal-postmenopausal groups and perimenopausal-postmenopausal groups for tissue 8-OHdG levels. No significant correlations were found between various measure of periodontal disease and GCF/tissue 8-OHdG levels among all the groups.
Premenopausal-postmenopausal and perimenopausal-postmenopausal transition resulted in significant increase in tissue and GCF 8-OHdG levels. However, no association was found between stages of reproductive ageing and tissue levels of 8-OHdG.
本研究旨在评估患有慢性牙周炎的绝经前、围绝经期和绝经后女性的组织及龈沟液（GCF）中氧化应激标志物8-羟基-2'-脱氧鸟苷（8-OHdG）的水平。
氧化应激与牙周炎的发病机制有关，且绝经会引发氧化应激。
根据生殖衰老分期研讨会（STRAW）标准，将诊断为牙周炎的女性分为三组，每组31名参与者：1. 绝经前；2. 围绝经期；3. 绝经后。从探诊深度最大的部位采集GCF和牙龈组织样本。从牙龈样本中提取组织DNA，并使用酶联免疫吸附测定法（ELISA）测量提取的DNA及GCF样本中的8-OHdG。
三组之间GCF中8-OHdG的总体水平存在高度显著差异，绝经前-绝经后组和围绝经期-绝经后组之间的两两差异高度显著。然而，三组之间组织8-OHdG水平未发现总体显著差异。在绝经前-绝经后组和围绝经期-绝经后组之间，组织8-OHdG水平存在两两高度显著差异。在所有组中，未发现牙周疾病的各种测量指标与GCF/组织8-OHdG水平之间存在显著相关性。
绝经前-绝经后和围绝经期-绝经后转变导致组织和GCF中8-OHdG水平显著升高。然而，未发现生殖衰老阶段与组织8-OHdG水平之间存在关联。
