Rennick D M, Lee F D, Yokota T, Arai K I, Cantor H, Nabel G J
J Immunol. 1985 Feb;134(2):910-4.
We recently isolated from a mouse T cell cDNA library a full-length clone that encodes a mast cell growth factor (1). On the basis of sequence homologies, this cloned factor must be similar if not identical to purified interleukin 3 (IL 3) (2, 3). Here, we report the first biologic characterization of the cloned gene product expressed in COS-7 monkey cells. Our results establish that a single molecular species promotes the growth and differentiation of a wide spectrum of hematopoietic cell types, including multipotential stem cells and various committed progenitor cells. The relationship of cloned IL 3 to other colony-stimulating factors is discussed.
我们最近从小鼠T细胞cDNA文库中分离出一个编码肥大细胞生长因子的全长克隆（1）。根据序列同源性，这个克隆的因子即便与纯化的白细胞介素3（IL-3）不完全相同，也必定相似（2,3）。在此，我们报告在COS-7猴细胞中表达的克隆基因产物的首次生物学特性分析。我们的结果表明，单一分子种类可促进多种造血细胞类型的生长和分化，包括多能干细胞和各种定向祖细胞。文中还讨论了克隆的IL-3与其他集落刺激因子的关系。
