Zhang Yun, Zhang Xiangwen, Yang Xiao-Feng, Zhang Juan
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an, People's Republic of China.
Chemical Research Institute, School of Chemical Engineering, Northwest University, Xi'an, People's Republic of China.
Luminescence. 2017 Nov;32(7):1233-1239. doi: 10.1002/bio.3316. Epub 2017 Jun 1.
A coumarin-benzopyrylium (CB) platform has been developed for the colorimetric and fluorogenic detection of bisulfite. The proposed probe utilizes coumarin as the fluorophore and positively charged benzopyrylium as the reaction site. The method employs the nucleophilic addition of bisulfite to the benzopyrylium moiety of CB to inactivate the electron-deficient oxonium ion. The driving force for photo-induced electron transfer is considerably diminished, thereby promoting the emission intensity of the coumarin fluorophore. The fluorescence intensity at 510 nm is linear with bisulfite concentration over a range of 0.2-7.5 μM with a detection limit of 42 nM (3δ). CB shows a rapid response (within 30 s) and high selectivity and sensitivity for bisulfite. Preliminary studies show that CB has great potential for bisulfite detection in real samples and in living cells.
已开发出一种香豆素 - 苯并吡喃鎓（CB）平台用于比色法和荧光法检测亚硫酸氢盐。所提出的探针利用香豆素作为荧光团，带正电荷的苯并吡喃鎓作为反应位点。该方法利用亚硫酸氢盐对CB的苯并吡喃鎓部分进行亲核加成，使缺电子的氧鎓离子失活。光诱导电子转移的驱动力大大减弱，从而提高了香豆素荧光团的发射强度。在0.2 - 7.5μM范围内，510nm处的荧光强度与亚硫酸氢盐浓度呈线性关系，检测限为42 nM（3δ）。CB对亚硫酸氢盐显示出快速响应（30秒内）以及高选择性和灵敏度。初步研究表明，CB在实际样品和活细胞中亚硫酸氢盐检测方面具有巨大潜力。
