提取物调节的信号通路抑制大鼠脑中晚期糖基化终末产物的形成。
SIGNALING PATHWAYS REGULATED BY EXTRACT INHIBIT THE FORMATION OF ADVANCED GLYCATED END PRODUCTS IN RAT BRAIN.
作者信息
Al-Malki Abdulrahman L, Barbour Elie K, Ea Huwait, Moselhy Said S, ALZahrani Anas Hassan Saeed, Kumosani Taha A
机构信息
Department of Biochemistry, Faculty of Science, King Abdulaziz University (KAU), P.O Box 80203, Jeddah, Saudi Arabia.
Bioactive Natural Products Research Group, KAU.
出版信息
Afr J Tradit Complement Altern Med. 2017 Jan 13;14(2):234-240. doi: 10.21010/ajtcam.v14i2.25. eCollection 2017.
BACKGROUND
The goal of this study was identification signaling molecules mediated the formation of AGEs in brain of rats injected with CdCl2 and the role of camel whey proteins and extract on formation of AGEs in brain.
METHODS
Ninety male rats were randomly grouped into five groups; Normal control (GpI) and the other rats (groups II-V) were received a single dose of cadmium chloride (5 μg/kg/b.w) for induction of neurodegeneration. Rats in groups III-V were treated daily with whey protein (1g/kg b.w) or extract (1mg/kg b.w) or combined respectively for 12 weeks.
RESULTS
It was found that whey protein combined with extract prevented the formation of AGEs and enhance the antioxidant activity compared with untreated group (p <0.001). Serum tumor necrosis factor (TNF-α) and interleukine (IL-6) levels were significantly decreased (p<0.01) in rats treated with whey protein and extract formation compared with untreated. The combined treatment showed a better impact than individual ones (p<0.001). The level of cAMP but not cGMP were lowered in combined treatment than individual (p<0.01).
CONCLUSION
It can be postulated that Whey protein + extract formation could have potential benefits in the prevention of the onset and progression of neuropathy in patients.
背景
本研究的目的是确定介导注射氯化镉的大鼠大脑中晚期糖基化终产物（AGEs）形成的信号分子，以及骆驼乳清蛋白和提取物对大脑中AGEs形成的作用。
方法
将90只雄性大鼠随机分为五组；正常对照组（第一组），其他大鼠（第二至五组）接受单剂量氯化镉（5微克/千克体重）以诱导神经退行性变。第三至五组大鼠分别每天用乳清蛋白（1克/千克体重）或提取物（1毫克/千克体重）或两者联合处理12周。
结果
发现与未处理组相比，乳清蛋白与提取物联合使用可防止AGEs的形成并增强抗氧化活性（p<0.001）。与未处理组相比，用乳清蛋白和提取物处理的大鼠血清肿瘤坏死因子（TNF-α）和白细胞介素（IL-6）水平显著降低（p<0.01）。联合处理比单独处理显示出更好的效果（p<0.001）。联合处理组中cAMP水平降低，但cGMP水平未降低，与单独处理组相比有差异（p<0.01）。
结论
可以推测，乳清蛋白+提取物联合使用可能对预防患者神经病变的发生和发展具有潜在益处。
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