Department of Biochemistry, University of Allahabad, Allahabad, 211002, India.
Department of Zoology, Savitribai Phule Pune University, Pune, 411007, India.
Biol Futur. 2020 Sep;71(3):273-281. doi: 10.1007/s42977-020-00033-5. Epub 2020 Jul 28.
Aging is associated with decreased cellular cysteine uptake, which acts as a precursor for glutathione biosynthesis. Whey protein, a liquid aspect of milk, is an effective cysteine delivery system. The study was undertaken to evaluate the potential role of whey protein concentrate (WPC) on the redox biomarkers during aging. Male Wistar rats were divided into following four groups: young control (4 months old); young treated with WPC (300 mg/kg b.w./day orally); old (24 months old) control; old treated with WPC for 28 days. After treatment, changes in body weight, lipid profile and levels of redox biomarkers were determined. A marked decrease in prooxidants such as reactive oxygen species, lipid peroxidation and protein carbonyl and significant (p ≤ 0.05) increase in antioxidants such as reduced glutathione and GST levels were observed after WPC supplementation in old age rats. We also found marked decrease in the level of sialic acid and AGEs after WPC supplementation. In conclusion, WPC provides protection against age-dependent redox imbalance which might be attributed to its antioxidant activity.
衰老是与细胞半胱氨酸摄取减少有关的，半胱氨酸是谷胱甘肽生物合成的前体。乳清蛋白是牛奶的液体部分，是一种有效的半胱氨酸输送系统。本研究旨在评估乳清蛋白浓缩物（WPC）在衰老过程中对氧化还原生物标志物的潜在作用。雄性 Wistar 大鼠分为以下四组：年轻对照组（4 个月大）；年轻 WPC 处理组（300mg/kg b.w./天口服）；老年对照组（24 个月大）；WPC 处理 28 天的老年组。治疗后，测定体重变化、血脂谱和氧化还原生物标志物水平。在老年大鼠中补充 WPC 后，观察到促氧化剂（如活性氧、脂质过氧化和蛋白质羰基）显著减少，而抗氧化剂（如还原型谷胱甘肽和 GST 水平）显著增加（p≤0.05）。我们还发现，补充 WPC 后唾液酸和 AGEs 的水平明显下降。结论：WPC 提供了对抗年龄相关氧化还原失衡的保护，这可能归因于其抗氧化活性。
